摘要
从虎杖内生细菌和黏细菌中筛选吡咯喹啉醌(PQQ)产生菌。采用3种以甲醇为唯一碳源的培养基对160株供试菌株进行摇瓶培养发酵,发酵产物采用光谱学分析法及HPLC法筛选。结果显示,通过初筛和复筛共得到甲醇利用型菌134株,PQQ产生菌4株,其中菌株083114的PQQ产量为64.34 mg/L。菌株083114的16S rRNA基因序列分析结果显示,其序列与酸快生芽孢杆菌(Bacillus acidiceler)的系统发育关系最近。虎杖内生细菌及黏细菌中存在PQQ产生菌。
The aim was to isolate Pyrroloquinolirie quinine producing strains from endophytic bacteria of Polygonum cuspidatum and strains of myxobacteria. Three kinds of media with methanol as single carbon source were used for microbial fermentation of the strains. Spectroscopy and HPLC analysis of the fermentation product were performed. Through preliminary screening and complex screening we got 134 strains of methanol-utilizing and 4 strains of Pyrroloquinoline quinine producing. The Pyrroloquinoline quinine producing strain 083114 was determined to be 64.34 mg/L. The 16S rRNA sequence analysis showed that the phylogenetic relationship between strain 083114 and Bacillus acidiceler is the most similar. Therefore, it proved that Pyrroloquinoline quinine producing bacteria were in endophytic bacteria of Polygonum cuspidatum and myxobacteria.
出处
《生物技术通报》
CAS
CSCD
北大核心
2013年第1期162-165,共4页
Biotechnology Bulletin
关键词
甲醇利用型菌
吡咯喹啉醌
内生细菌
黏细菌
光谱法
Methanol-utilizing bacteria Pyrroloquinoline quinine Endophytic bacteria Myxobacteria Spectroscopy
