摘要
为快速检测猪伪狂犬病毒(PRV),利用PRV DBP基因上的一段序列设计并合成3对LAMP引物,采用一种新的荧光染料——罗丹明B衍生物作指示剂,该指示剂在反应前加到反应缓冲液里,通过优化反应条件,建立了可视化LAMP快速检测PRV的方法。结果表明,63℃下恒温反应40min可得到肉眼可视结果,颜色变成蓝色判定为阳性,仍然保持紫色判定为阴性,可视化LAMP结果与电泳结果一致。建立的可视化LAMP方法可以快速、直观、准确地检测PRV。
Three pairs of primers were designed according to conservative sequences on DBP genes on Pseudorabies virus（PRV）.A method of loop-mediated isothermal amplification（LAMP） for rapid and visible detection of PRV has been established by optimizing reacting system using rhodamine B derivatives as the indicator.The results showed that the visible results can be observed at 40 min under the condition of 63 ℃ water bath.The blue color is regarded as positive while the purple is negative.The result of visible LAMP was in accordant with that of agarose gel electrophoresis.
出处
《河南农业科学》
CSCD
北大核心
2012年第6期140-143,共4页
Journal of Henan Agricultural Sciences
基金
现代农业产业技术体系建设专项资金资助项目(nycytx 009)
