摘要
壳聚糖经果糖修饰以改善其水溶性,再经半胱氨酸修饰得到巯基化果糖-壳聚糖。海藻酸钠与巯基化果糖-壳聚糖混合溶液经氯化钙及硫酸钠双重交联制得复合水凝胶珠。溶胀试验结果表明:该凝胶珠在pH值6.8及7.4时的溶胀率分别是pH值1.2时的7倍和10倍左右。牛血清白蛋白(BSA)包载试验结果表明:BSA重量为凝胶珠质量的20%时,包载率可达94%以上,随着巯基化果糖-壳聚糖在凝胶珠中比例增加,BSA包载率上升。BSA释放试验表明:pH值1.2时BSA的释放率很低,只有6%～10%的BSA从凝胶珠中释放出来,随后累积释放量基本不变;pH值6.8和pH值7.4时BSA的释放率迅速提高,因此这种复合水凝胶珠可作为一种潜在的口服蛋白类药物载体。
Chitosan was modified by fi＇uctose for improving its solubility in water, and then thiolated fructose-modified chitosan was pre- pared by cysteine modification. The composite hydrogel of alginate/thiolated fructose-modified chitosan was prepared according to the following three steps： 1. the sodium alginate solution and thiolated fructose-modified ehitosan solution were respectively prepared and then mixed; 2. the mixed solution was crosslinked by calcium ion; 3. the calcium crosslinked gel beads were again crosslinked by sodium sulfate solution to form the composite crosslinked hydrogel beads. The experimental results indicated that the swelling ratios of composite crosslinked hydrogel beads in pH 6. 8 and pHT. 4 were about 7 and 10 times higher than they in pH 1.2, respectively ; that loading efficiency of bovine serum albumin （ BSA ） on composite crosslinked hydrogel beads exceeded 94% when the weight ratio of BSA to polymer was 20% ; and that the loading efficiency of BSA on composite crosslinked hydrogel beads seemed to increase with in- creasing the amount of tbiolated fructose-modified cbitosan in formulation of hydrogel beads. The amount of BSA released from the beads at pH 1.2 was relatively low （about 6% -10% ）. Thereafter, the cumulative release of BSA kept almost no variety. But at pH 6. 8 and 7.4, the release amount of BSA increased rapidly. The composite hydrogel beads based on alginate/thiolated fructose-modified chitosan may be a potential polymeric carrier for oral delivery of protein drugs.
出处
《生物学杂志》
CAS
CSCD
2013年第1期22-26,17,共6页
Journal of Biology
