摘要
目的建立一个稳定的兔宫腔粘连(IUA)动物模型,以利于深入探讨宫腔粘连的发病机制。方法 60只雌兔随机分为对照组、机械损伤组、感染损伤组和双重损伤组,每组15只。机械损伤组刮宫损伤子宫内膜,感染损伤组宫腔留置脂多糖棉线2d损伤子宫内膜,双重损伤法刮宫后宫腔留置脂多糖棉线2d损伤子宫内膜,对照组行假手术。分别于损伤前,损伤后2、7、14、28d收集子宫组织,行HE和Masson染色,动态观察子宫内膜病理变化过程,并对损伤后7d子宫内膜的腺体数量和纤维化面积进行统计学分析。结果双重损伤组子宫内膜纤维化面积比率(0.743±0.066)高于机械损伤组和感染损伤组(0.359±0.061 4、0.348±0.086),分别比较,差异有统计学意义(P<0.05)。双重损伤组兔子宫内膜腺体数量(4.190±1.350)低于另外2组(12.660±1.470、13.590±1.560),分别比较,差异均有统计学意义(P<0.05)。结论采用机械和感染双重损伤法可以建立稳定的兔宫腔粘连动物模型。
Objective To construct a rabbit model of intrauterine adhesions（IUA） to benefit the deepening study Of intrauterine adhesions pathogenesis. Methods Sixty New Zealand female rabbits were randomly divided into the control group,mechanical inju- ry group,infection injury group and double-injury group, 15 cases in each group. The mechanical injury group was treated with cu- rettage of uterine, the infection injury group with intrauterine indwelling lipopolysaccharide cotton for 2 d, the double damage group with indwelling lipopolysaccharide cotton for 2 d after curettage for injuring endometrium and the control group was performed the sham-operation. In order to dynamically observe the endometrial pathological changes process,the uterine tissues were collected re- spectively before modeling,on 2,7,14,28 d after modeling and performed HE and Masson staining. The number of endometrial glands and the ratio of fibrosis area on 7 d after modeling in each group were analyzed by statistical software. Results The ratio of fibrosis area in the double-injury group was 0. 743±0. 066 and higher than 0. 359±0. 061 4 and 0. 348±0. 086 in the mechanical in- jury group and the infection group respectively,showing statistical differences between them（P〈0.05）. The number of endometrial glands in the double-injury group was 4. 190±1. 350 and lower than 12. 660±1. 470 and 13. 590±1. 560 in other two groups with statistical differences（P〈0.05）. Conclusion The double damage method of mechanical injury and infection can establish stable rabbit uterine adhesion models.
出处
《重庆医学》
CAS
CSCD
北大核心
2013年第7期765-767,共3页
Chongqing medicine
基金
国家自然科学基金资助项目(81270658)
关键词
宫腔粘连
动物模型
损伤
纤维化
intrauterine adhesions
animal models
injury
fibrosis
