摘要
以壳聚糖为唯一碳源,结合固态培养基发酵培养,从自然界中筛选得到一株产复合酶的菌株,初步鉴定为曲霉属。该菌产复合酶中以壳聚糖酶、纤维素酶和α-淀粉酶为主,它们的酶活分别为2.206、0.790、3.229U/mL。该复合酶降解壳聚糖的最适温度为50℃,最适pH为5.6,最适时间为4h。通过有机溶剂分级沉淀,发现该复合酶降解壳聚糖的最终产物以聚合度为8～16的壳寡糖为主,表明该复合酶在壳寡糖制备方面具有一定的应用价值。
A complex enzyme production strain for chitosan biodegradation was isolated from soil based on the method of using chitosan as the sole carbon source and solid fermentation medium as the second screening medium.And the strain is identified as Aspergillus based on its morphology observation.Chitosanase,cellulase and co-amylase were the main components in complex enzymes, and the activity of these enzymes was 2.206,0.790, 3.299U/mL,respectively. Moreover, the optimal conditions for chitosan biodegradation was 50℃, pH5.6 and the optimal operate time was 4h based on the measuring of reducing sugar produced from chitosan hydrolyzation. Finally,the potential use of this complex enzyme in preparation of chitooligosaccharides was confirmed by organic solvent precipitation method.
出处
《食品工业科技》
CAS
CSCD
北大核心
2013年第5期172-176,共5页
Science and Technology of Food Industry
基金
湖北省教育厅项目(B20114607)
武汉市教育局项目(2010085)
关键词
壳聚糖
壳寡糖
复合酶
筛选
降解
chitosan
chitooligosaccharide
complex enzyme
screen
degradation
