摘要
ECE支TCP基因调控植物的分枝和花的对称性,但其功能在不同物种间存在差异。利用简并PCR法结合RACE技术从矮牵牛(Petunia hybrida Vilm)GL8自交系中获得了4个矮牵牛ECE支TCP基因家族成员的全长cDNA序列,分别命名为PhTCP1～4(登录号:JQ400104～JQ400107)。cDNA和基因组序列分析表明,PhTCP1～4分别编码406、332、341和343个氨基酸,PhTCP1不含内含子,其余3个基因含1～2个内含子。进化树分析发现,PhTCP1～4分属于CYC1、CYC2和CYC3分支。荧光定量PCR分析表明,PhTCP1～4均在成株期矮牵牛腋芽中优势表达,在不同节位腋芽中表现的表达趋势各不相同,提示矮牵牛ECE支TCP基因可能主要与腋芽的生长发育相关。
The TCP family is an ancient group of plant developmental transcription factors that regulate cell division in vegetative and reproductive structures. The ECE clade TCP genes include genes mainly involved in the development of axillary meristems giving rise to either flowers or lateral shoots. They play critical roles in plant evolution and crop domestication process. In order to study the function of ECE clade of petunia, degenerate primer PCR method and RACE （rapid amplification of cDNA ends） method were used to isolate the ECE clade TCP genes from petunia. Four full-length cDNA sequences ~nd their corresponding gnomic sequences were obtained, and designated as PhTCP1, PhTCP2, PhTCP3 and PhTCP4 respectively. The GenBank accession numbers for cDNA are JQ400104 -JQ400107. Genomic DNA and cDNA analyses showed that PhTCP1 - 4 genes encode proteins of 406, 332,341 and 343 amino acids respectively. The PhTCP1 is a gene without intron, while the others have 1 - 2 introns. Phylogenetic analysis indicated that PhTCP1 -4 genes belong to the CYC1, CYC2 and CYC3 clade respectively. Real-time quantitative PCR showed that PhTCP1 - 4 genes were expressed mainly in were expressed mainly were expressed mainly in axillary buds of mature petunia, suggesting that the ECE clade TCP genes may be primarily associated with the growth and development of axillary buds.
出处
《园艺学报》
CAS
CSCD
北大核心
2013年第2期307-316,共10页
Acta Horticulturae Sinica
基金
国家自然科学基金项目(31272199)
