摘要
目的通过体外实验观察顺铂联合热疗对人胃癌耐药细胞增殖及多药耐药基因GST-π表达的影响。方法选取人胃癌SGC7901/DDP细胞系进行体外培养。应用流式细胞仪(flow cytometry,FCM)检测对照组、化疗组、热疗组及热化疗组SGC7901/DDP细胞增殖的情况,并用FCM法半定量检测各组细胞中GST-π蛋白表达的变化;采用逆转录-聚合酶链反应(RT-PCR)法半定量检测上述四组SGC7901/DDP细胞中GST-πmRNA表达的变化。结果热疗及顺铂联合热疗都可以降低人胃癌耐药细胞SGC7901/DDP的增殖水平,并且顺铂联合热疗作用效果最明显。单纯热疗及顺铂联合热疗作用后,SGC7901/DDP细胞中GST-π蛋白及mRNA的表达明显的下降,并且并顺铂联合热疗作用后下降最明显。结论热疗、顺铂联合热疗能够抑制人胃癌耐药细胞株SGC7901/DDP的增殖,并可诱导该细胞凋亡;下调GST-π蛋白及mRNA的表达可能是热疗逆转人胃癌耐药细胞株SGC7901/DDP多药耐药性的机制之一。
[ Objective ] To observe the effects of cisplatin combined with hyperthermia on human drug resistant gastric cancer cell proliferation and muhidrug resistance gene expression of GST-π by in vitro experiments. [Method] Human gastric cancer SGC7901/DDP cell lines were cultured in vitro. SGC7901/DDP cell proliferation was detected by flow cytometry （FCM） in control group, chemotherapy group, hyperthermia group and the thermochemotherapy group. Semi-quantitative FCM method was used to detect changes in the expression of GST-π in cells in each group; Semi-quantitative RT-PCR was used to detect changes in the expression of GST-π mRNA in the four groups SGC7901/DDP cells. [Results] Both hyperthermia and cisplatin combined with hyperthermia can reduce the level of proliferation of SGC7901/DDP. GST-＇~ protein and mRNA expression in SGC7901/DDP ceils were significantly decreased after hyperthermia or cisplatin combined with hyperthermia. Cisplatin combined with hyperthermia is more effective. [ Conclusion ] Both hyperthermia and cisplatin combined with hyperthermia could inhibit the proliferation of SGC7901/DDP, and induce cell apoptosis and inhibit cell cycle induction of apoptosis. Down GST-πprotein and mRNA expression may be one of the multidrug resistance mechanism of hyperthermia reversal of drug- resistant SGC7901/DDP cell lines.
出处
《中国现代医学杂志》
CAS
CSCD
北大核心
2012年第34期14-17,共4页
China Journal of Modern Medicine
基金
河北省重点科技研究计划(No:20110445)
关键词
热疗
热化疗
胃癌耐药细胞
逆转耐药
GST-Π
hyperthermia
thermo-chemotherapy
multidrug resistance gastric cell line
reverse multiple drug re- sistance
GST-π
