摘要
目的:对手足口病(HFMD)进行病原学监测,了解北京市西城区HFMD的流行特征。方法:提取标本中病毒核酸,采用荧光RT-PCR方法进行核酸鉴定,核酸阳性标本使用RD细胞进行病毒分离培养。结果:2011年共收集咽拭子标本192份,检测结果显示,肠道病毒核酸阳性81例,阳性率为42.19%。EV71、CoxA16及未分型肠道病毒核酸阳性分别为32例(16.67%)、36例(18.75%)和13例(6.77%);病毒分离培养过程中我们对标准方法中的操作进行了改良,培养阳性率达到91.23%。结论:2011年北京市西城区手足口病病原以CoxA16和EV71为主;手足口病毒分离的改良培养方法操作方便,不仅可以提高阳性率,而且增加获得的病毒量。
Objective：Based on pathogen monitoring of hand, foot and mouth disease （Hand Foot and Mouth Disease, HFMD）, to understand the epidemiological characteristics of HFMD in Xicheng District, Beijing. Meth- ods： The viral nucleic acid was extracted from HFMD specimens, and identified by fluorescent RT - PCR, then the positive specimens were isolated and cultured with RD cell. Results： In 2011, eighty - one specimens were found enterovirus nucleic acid positive from 192 throat swabs（42.19% ）, including 32 EV71 cases（ 16.67% ）, 36 Cox- A16 cases（ 18.75% ） and 13 untyped enterovirus cases（6.77% ）. Virus isolation results showed that the positive rate of culture reached 91.23% with improved method. Conclusion： In 2011, CoxA16 and EV71 were the domi- nant pathogens for HFMD in Xicheng district of Beijing, the improved culture method for HFMD virus isolation is easy to operate, with high positive detection rate and viral load.
出处
《中国卫生检验杂志》
北大核心
2013年第2期460-462,共3页
Chinese Journal of Health Laboratory Technology
关键词
手足口病
RT—PCR
病毒分离
Hand, foot and mouth disease
RT-PCR
Virus isolation
