摘要
[目的]建立反相高效液相色谱法对杀草悬乳剂中杀草敏含量进行快速分析。[方法]使用配备了瑞典Kromasil 100-5-C18不锈钢色谱柱的日本岛津10-A VP Plus高效液相色谱仪,利用高效液相色谱法测定杀草悬乳剂中杀草敏的含量,选用体积比为40:60的甲醇-水溶液为流动相;流速控制在1.0 mL/min;柱温:室温(要求实验过程中温差变化小于2℃);检测波长286 nm;进样体积20μL。[结果]在1～100 mg/L的范围内杀草敏线性关系良好(线性相关系数0.999852),平均回收率为100.52%,RSD=0.298%。[结论]此方法可快速测定杀草悬乳剂中杀草敏的含量,具有快速、简便、准确、精密度好,方法新颖等优点。
A method for rapid determination of pyrazon content in Herbicide suspended emulsions was developed by RP-HPLC. That is： SHIMADZU 10-A VP Plus HPLC, Sweden Kromasil 100-5-C18 stainless steel HPLC column, mobile phase： methanol ＋ water solution （40：60, by volume）; flow rate： 1.0 mL/min; col- umn temperature： room temperature （temperature changes during experiment process is less than 2 ℃）; de- tection wavelength： 286 nm; injection volume 20 μ L. Results showed that a good linear relationship when pyrazon at 1-100 mg/L, the average recovery was 100.52%, RSD was 0.298%. This method can be used for determine pyrazon content in suspended emulsions with the characteristics of fast, simple and accurate, pre- cise, novel means.
出处
《吉林农业科学》
CSCD
北大核心
2013年第1期27-29,共3页
Journal of Jilin Agricultural Sciences
基金
江苏省教育厅文件(苏教高[2008]38号)
