摘要
采用分子印迹技术 ,以 α-甲基丙烯酸为功能单体 ,二甲基丙烯酸乙二醇酯为交联剂 ,合成出以奎宁为模板的分子印迹聚合物。利用荧光法研究了聚合物的吸附性能和选择识别能力。结果表明 ,和化学组成相同的空白聚合物相比 ,印迹聚合物具有高吸附能力和选择识别能力。奎宁分子印迹聚合物与奎宁分子的离解常数达 1 .0 8× 1 0 - 3mol/L,表观最大吸附量为 1 31 .8μmol/g,为理论值的 56.4%。
The molecularly imprinted polymer with alkaloid quinine as template was prepared by molecular imprinting technique using α methacrylic acid as the functional monomer and ethylene glycol dimethacrylate as the crosslinker. The absorption property and selective recognition ability of the new polymer were studied by fluorescent method. The result showed that the polymer had higher absorption and higher selective recognition ability compared to the blank polymer which had the same chemical composition. The dissociation constant of the imprinted polymer to the template quinine was estimated to be 1.08×10 -3 mol/L. And the apparent maximum number of binding sites of the imprinted polymer was to be 131.8 μmol/g which therefore correspond to 56.4% of the theoretical total binding sites derived from the amount of the template used for the polymerization. It indicated that this was a new way for the selective enrichment and determination of trace quinine in biosamples.
出处
《分析科学学报》
CAS
CSCD
2000年第4期310-313,共4页
Journal of Analytical Science
关键词
分子印迹
奎宁
印迹聚合物
选择性识别
吸附
Molecular imprinting
Quinine
Imprinted polymer
Selective recognition
Absorption
