摘要
背景:气管支架制备是组织工程学方法修复长段气管缺损的关键步骤。目的:通过比较分析3种制备异体脱细胞气管支架的方法,为组织工程气管支架制备寻找更适宜的途径。方法:手术获得兔新鲜气管,分为对照组、玻璃化液冷冻法组、酶洗法组、改良玻璃化液冷冻法组。处理后对各组标本行苏木精-伊红染色,电镜扫描观察,并测量气管最大拉伸力、破裂力和组织拉伸率等生物力学性能。结果与结论:组织学观察显示对照组、玻璃化液冷冻法组可见部分完整黏膜上皮细胞,酶洗法组、改良玻璃化液冷冻法组未见黏膜上皮细胞。电镜观察示对照组、玻璃化液冷冻法组、改良玻璃化液冷冻法组有丰富的细胞外基质和胶原纤维,而酶洗法组无细胞外基质,只有胶原纤维。组间两两比较,气管支架的最大拉伸力、最大破裂力和组织拉伸率比较,差异均无显著性意义。说明应用改良玻璃化液冷冻法制备气管支架能够有效地去除抗原性、保留细胞外基质,并维持生物力学性能,是一种较为理想的组织工程气管支架制备方法。
BACKGROUND： It is a key step to prepare tracheal matrices in tissue engineering for repairing long segment tracheal defects.OBJECTIVE： To find the better method for preparing tissue engineering tracheal matrices through comparing and analyzing three different ways of preparing allogeneic acellular tracheal matrices.METHODS： Fresh rabbit tracheas were obtained by surgical operation and divided to four groups： untreated group, conventional vitrificational group, detergent-enzymatic group, modified vitrificational group. Hematoxylin-eosin staining and scanning electron microscopy were used to observe the morphology and ultrastructure of the treated tracheal matrices. The biomechanical properties including maximum tensile force, rupture force and tissue elongation of the treated tracheal matrices were measured.
RESULTS AND CONCLUSION： Histological observations showed that there were epithelial cells in the untreated and conventional vitrificational groups, but there were no epithelial cells in the detergent-enzymatic and modified vitrificational groups. There were abundant extracellular matrices and collagen fibers in the untreated group, conventional vitrificational group, and modified vitrificational group under electromicroscopy. Conversely, no extracellular matrix was found in the detergent-enzymatic group, in which there were only collagen fibers. Finally, no statistical differences were found in the maximum tensile force, rupture force and tissue elongation among all the groups. The method of modified vitrification, by which the antigen can be removed, and the extracellular matrix and biomechanical properties can be maintained effectively, is a more ideal way to prepare tissue engineering tracheal matrix.
出处
《中国组织工程研究》
CAS
CSCD
2013年第2期191-195,共5页
Chinese Journal of Tissue Engineering Research
关键词
组织构建
组织构建基础实验
气管支架
酶洗法
玻璃化液冷冻法
改良的玻璃化液冷冻法
细胞外基质
组织构建图片文章
tissue construction
basic experiments of tissue construction
tracheal scaffold
enzymatic washing conventional vitrificational method
modified vitrificational method
extracellular matrix
tissue construction photographs-containing paper
