摘要
目的 研究分析在不同级别脑胶质瘤细胞端粒酶活性的表达及端粒长度的变化。方法 采集 40例脑胶质瘤手术切除标本、 4例正常脑组织 ,通过半定量端粒重复序列扩增 (telomererepeatamplificationprotocol,TRAP) 银染方法检测端粒酶活性水平 ,应用人的端粒序列特异性探针32 P (CCC TAA) 3 进行Southern杂交检测脑胶质瘤细胞的端粒长度。结果 在 40例胶质瘤标本中的 33例(82 5 % )中均检出端粒酶活性 ,而在正常脑组织中无端粒酶活性的表达 ,不同级别胶质瘤之间端粒酶活性水平有明显差异 ;恶性胶质瘤细胞中端粒的长度明显比正常胶质细胞缩短 ,端粒的长度与端粒酶活性的水平有着显著的的负相关。结论 端粒酶活性可以作为脑胶质瘤的恶性标记之一 ,端粒的缩短可能是脑胶质瘤进展的重要因素 。
Objective To investigate the relationship of telomerase activity and telomere length in different grades of glioma.Methods 40 rapidly specimens of surgical resected glioma and 4 normal brain tissue were used to study the telomerase activity by semi quantitative telomeric repeat amplification protocol (TRAP) silver staining. Telomere length was estimated from southern blots of telomere restriction fragments (TRFs)with 32 P (CCCTAA) 3 probe specific for human telomeric sequences.Results Telomerase activity was detected positively in 33 of 40(82 5%)gliomas specimens, and none of normal brain tissue. The level of telomerase expression was associated with grade of tumor. The length of telomerase in maligant gliomas was shorter than that in normal glia cells. The length of also telomere correlated with telomerase activity. Conclusions Our findings suggest that telomerase activity could be a special marker of gliomas and an index of maligant potential. Telomere reduction is one of the important genetic events during transformation of gliomas. Telomere repair mechanism may be initiated in glioma which may maintain a relative stability of TRFs and permit a constitutive proliferation of these malignant cells.
出处
《中华神经外科杂志》
CSCD
北大核心
2000年第5期316-319,共4页
Chinese Journal of Neurosurgery
