摘要
目的评价苦参胶囊的抗炎镇痛作用.方法记录小鼠24 h湿粪总数,观察苦参胶囊对番泻叶所致小鼠泄泻的影响,以炭末法评价其对正常小鼠小肠运动的影响.采用试管两倍稀释法观测苦参胶囊在体外对痢疾杆菌、大肠杆菌、伤寒杆菌、金黄色葡萄球菌等致病菌的影响;小鼠耳廓肿胀、大鼠足跖肿胀模型,观测苦参胶囊抗炎消肿作用,同时检测苦参胶囊对小鼠腹腔毛细血管通透性的影响.运用小鼠热板法及醋酸扭体方法评价苦参胶囊的镇痛作用.结果 1和2 g/kg的苦参胶囊均明显减少番泻叶所致泄泻的湿粪总数;苦参胶囊中、高剂量组的小肠推进距离均明显减小.扭体试验表明中、高剂量的苦参胶囊明显较少小鼠扭体次数,热板法显示1和2g/kg的苦参胶囊于给药后60～120 min均显著延长小鼠置入圆筒至开始舔后足时间.小鼠耳廓肿胀法表明高、中剂量的苦参胶囊均明显减轻小鼠耳廓肿胀度;大鼠足跖肿胀模型显示中、高剂量的苦参胶囊于末次给药后1～4 h均可减轻足跖肿胀度,于给药后1 h起效,作用至少可持续4 h.高、中剂量的苦参胶囊显著降低小鼠腹腔毛细血管通透性,低剂量对腹腔液的吸光度均无明显影响.苦参胶囊对痢疾杆菌、大肠杆菌、伤寒杆菌、金黄色葡萄球菌的MIC分别为1.25 mg/mL、0.625 mg/mL、5 mg/mL、5 mg/mL.结论苦参胶囊可减轻腹泻和小肠推进,具有镇痛抗炎、抑菌作用并降低毛细血管通透性.
Objective To investigate the anti-inflammatory and ianalgesic effects of Sophora Flavescens capsule. Methods Total number of muck in mice with senna-induced diarrhea within 24 h was recorded to observe the influence of Sophora Flavescens capsule, and charcoal-powder method was used to evaluate the effect of Sophora Flavescens capsule on intestinal movement in normal mice. Effects of Sophora Flavescens capsule on pathogens such as Dysentery bacilli, E.coli, Salmonella typhi, and Staphylococcus anreus were determined by the method of broth two-fold dilution. The anti-inflammatory effects were determined in the models of the mouse ear swelling, carrageenin-challenged rat paw edema, as well as the permeability of abdominal capillary in mice, respectively. The analgesic effect was evaluated by the hot plate and the acetic acid induced twist in mice. Results 1 g/kg or 2 g/kg of Sophora flavescens capsules significantly decreased the total number of muck in a dose-dependant manner in mice with senna-induced diarrhea. High and moderate dose of Sophora Flavescens capsule could obviouslv decrease twist times of the mice. After the mice had been significantly prolonged the time that the medicated for 60-120 min, Sophora mice began to lick their hinds after Flavescens capsule （1 and 2 g/kg） putting into the cylinders. Sophora Flavescens capsule lightened the dimethylxylene-caused mouse ear swelling and carrageenin-challenged paw edema in rats, in a dose-dependent manner. Sophora Flavescens capsule decreased the capillary permeability in abdominal cavity of mice whereas had no influence on peritoneal fluid absorption. The minimal inhibitory concentrations （MIC） of Sophora Flavescens capsule on Dysentery bacilli, E.coli, Salmonella typhi, and Staphylococcus aureus were 1.25 mg/mL, 0.625 mg/mL, 5 mg/mL, and 5 mg/mL, respectively. Conclusion Sophora Flavescens capsule can reduce diarrhea and small intestinal propulsion, and has analzesic and anti-inflammatory effects_
出处
《昆明医科大学学报》
CAS
2013年第2期4-10,共7页
Journal of Kunming Medical University
基金
国家自然科学基金资助项目(81160401)
关键词
苦参胶囊
抗炎作用
镇痛作用
毛细血管通透性
抑菌作用
Sophora Flavescens capsule
Anti-inflammatory effect
Analgesic effect
Permeability of cap- illary
Bacteriostasis
