摘要
目的通过研究宫颈癌人端粒酶基因(Human telomerase RNA component gene,TERC gene)的表达情况,探讨宫颈癌与TERC基因异常表达的相关性,评估荧光原位杂交(Fluorescence in situ hybridization,FISH)技术检测TERC基因的表达对子宫颈上皮内瘤样病变(Cervical intraepithelial neoplasia,CIN)发展到宫颈癌的预测价值。方法采用FISH技术进行子宫颈上皮脱落细胞TERC基因的检测,实验对象81例,病理活检正常组20例,CIN1组28例,CIN2组12例,CIN3组9例,宫颈癌组12例。并进行宫颈癌与TERC基因相关性分析。结果在CIN1组、CIN2组、CIN3组和宫颈癌组中,TERC基因的表达均显著高于正常组(P<0.05);CIN1组和CIN2组之间无显著差异(P>0.05);CIN3组和宫颈癌组显著高于CIN1组(P<0.05);宫颈癌组显著高于CIN3组(P<0.05)。恶性程度越高,差异越显著(P<0.01)。结论 TERC基因异常表达与宫颈癌的发生发展密切相关。FISH检测TERC基因的方法有助于宫颈病变的早期筛查。
Objective： To explore the correlation between cervical cancer and the expression of human telomerase RNA component （TERC） gene and evaluate the significance of detecting TERC gene expression by fluo- rescence in situ hybridization （FISH） technique for predicting the progress from cervical intraepithelial neoplasia （CIN） to cervical cancer. Methods： TERC gene expression was detected by the method of FISH in uterine epithelial exfoliated cells from 81 patients including 20 patients in normal group, 28 patients in CIN1 group, 12 patients in CIN2 group, 9 patients in CIN3 group, and 12 patients in cervical cancer group. The correlation between cervical cancer and TERC gene was analyzed. Results： The positive rate of TERC gene test in normal group was significant lower than CIN1 group, CIN2 group （P〈0.05）, CIN3 group and cervical cancer group （P〈0.01） . There was no significant difference between CIN1 group and CIN2 group （P〉0.05） . There was also significant difference be- tween CIN3 group and cervical cancer group （P〈0. 05） . The higher malignant degree was, the more significant difference would be （P〈0. 01） . Conclusions： The abnormal expression of TERC gene is closely related with the process of cervical cancer. The FISH technique for detecting TERC gene may be an effective method for helping to diagnoseearly cervical cancer.
出处
《生殖医学杂志》
CAS
2013年第3期164-166,共3页
Journal of Reproductive Medicine
关键词
宫颈癌
人端粒酶基因
荧光原位杂交
早期筛查
Cervical cancer
Human telomerase gene
Fluorescence in situ hybridization
Ear-ly screening
