摘要
目的:观察微小RNA(miRNA)-19b在肝纤维化大鼠肝脏组织的表达,探讨miRNA-19b在肝纤维化进程中的作用。方法:将20只SD大鼠随机分成正常对照组和肝纤维化模型组。采用改良式复合因素法复制大鼠肝纤维化模型,分别在6、7周后,取各组大鼠肝脏组织,用荧光实时定量PCR方法检测miRNA-19b的表达。结果:6周后肝纤维化模型组大鼠肝脏组织miRNA-19b的表达低于正常对照大鼠组(P<0.05),7周模型组大鼠肝脏组织miRNA-19b的表达低于6周模型组(P<0.05)。结论:miRNA-19b在肝纤维化大鼠肝脏组织中表达降低,可能与纤维化进程相关,且可能成为肝纤维的诊断以及预后的一个指标。
Objective： To study the effects of miRNA-19b through observing the expression of miRNA-19b in the liver on hepatic fibrosis（HF） rats.Methods： 20 rats were randomly divided into the normal group,HF group.The HF model in rats was duplicated with Improved Composite Factor Method.The liver tissues were taken after 6 weeks and 8 weeks.MiRNA-19b was tested by florescent real-time quantitative PCR.Results： Compared to the normal control rats,the level of miRNA-19b was markedly decreased in HF rats after 6 weeks（P0.05）.besides that,the level of miRNA-19b was more prominently decreased in HF rats after 7 weeks than 6 weeks（P0.05）.Conclusions： MiRNA-19b was decreased in liver tissues of hepatic fibrosis rats.It probably involved in the progress of HF and was the marker of diagnosis and prognosis of HF.
出处
《现代生物医学进展》
CAS
2013年第4期611-612,606,共3页
Progress in Modern Biomedicine
基金
国家"十二五"重大专项(2012ZX10002003-004-006)
