摘要
目的:观察蒙成药给旺-9对四氯化碳诱导大鼠急性肝衰竭的影响。方法:采用灌胃法给30%四氯化碳(CCl4)(4 mL·kg-1)诱导大鼠急性肝衰竭模型。造模24 h时分别检测正常组、模型组、蒙成药给旺-9低、中、高剂量组(1.3,2.6,3.9g·kg-1)血清中丙氨酸转氨酶(ALT)和天冬氨酸转氨酶(AST)、一氧化氮(NO)、一氧化氮合酶(NOS)的活性以及肝指数和胃匀浆中的胃蛋白酶等指标。结果:模型组与正常组比较对血清AST,ALT均有极明显增高(均P<0.01);NO,胃蛋白酶和肝指数均有明显的增高(P<0.05)。各给药组与模型组比较,蒙成药给旺-9低剂量组对血清NO,胃蛋白酶均明显降低(均P<0.05)。蒙成药给旺-9中剂量对血清AST,ALT均明显降低(分别为P<0.01,P<0.05);NO,胃蛋白酶和肝指数也有明显降低(均P<0.05)。蒙成药给旺-9高剂量对血清AST,ALT均明显降低(分别为P<0.01或P<0.05);NO,胃蛋白酶和肝指数也有明显降低(P<0.05)。结论:蒙药祛"热"凉性方剂给旺-9对CCl4诱导的大鼠急性肝衰竭有明显的保护作用,中剂量组效果最佳。
Objective：To observe the effect of Mongolian medicine GEI Wang-9 on acute liver failure induced by carbon tetrachloride CCl4.Method：methods 30% carbon tetrachloride was used to induce model of acute liver failure.After 24 h,indexes alanine aminotransferase（ALT）,aspartate aminotransferase（AST）,nitric oxide（NO）,active of nitric oxide composite enzyme（NOS） of serum and liver index and pepsin in the stomach homogenate were detected in normal group,model group,Mongolian medicine GEI Wang-9 low,middle,high dose groups（1.3,2.6,3.9 g·kg-1）,respectively.Result：In the model group,compared with normal group,serum AST,ALT extremely increased significantly（all P0.01）;NO,nitric oxide composite enzyme and liver indexall increased significantly（P0.05）.Compared with model group,GEI Wang-9 low doses decreased significantly the serum NO,nitric oxide composite enzymeall（P0.05）.GEI Wang-9,middle doses decreased significantly the serum AST,ALT（P0.01,P0.0 5,respectively）;and NO,nitric oxide composite enzyme and liver index reduced significantly（P0.05）.High doses reduced significantly the serum AST,ALT（P0.01,P0.05,respectively）;NO,nitric oxide composite enzyme and liver index reduced significantly（P0.05）.Conclusion：Mongolian medicine GEI Wang-9 has protective actions on acute liver failure induced by carbon tetrachloride.
出处
《中国实验方剂学杂志》
CAS
北大核心
2013年第8期238-241,共4页
Chinese Journal of Experimental Traditional Medical Formulae
