摘要
目的:探讨microRNA-451(miR-451)在结直肠癌组织中的表达及其临床意义。方法:采用实时荧光定量聚合酶链反应(real-time qPCR)的方法在40例结直肠癌患者癌组织与切缘正常黏膜标本,以及8种结肠癌细胞株中检测miR-451的表达水平,并分析其与临床病理资料之间的关系。结果:与结直肠正常黏膜组织比较,miR-451在结直肠癌组织及各种结直肠癌细胞株中均呈明显的低表达(均P<0.05);按结直肠癌患者的不同临床病理特征分组比较发现,miR-451的表达差异与组织学类型和分化程度有关,在黏液性腺癌与低分化腺癌中表达水平最低(均P<0.05)。结论:miR-451在结直肠癌组织中呈低表达,且降低程度与结直肠癌分化程度及组织学类型相关。
Objective： To investigate microRNA-451 （miR-451） expression in colorectal cancer （CRC） tissues and its clinical significance. Methods： The miR-451 expression in tumor tissues and normal mucosa samples obtained from 40 CRC patients, as well as 8 different CRC cell lines were examined by real-time quantitative reverse transcription-PCR （RT-qPCR）. the relations of miR-451 expression with clinicopathologic profiles of CRC were analyzed Results： Compared with normal mucosal tissues, miR-451 expression level was significantly reduced in either CRC tissues or all the CRC cell lines （all P〈0.05）. Comparison between groups divided by clinicopathologic profiles showed that the miR-451 expression difference was associated with the histologic classification and degree of differentiation of the tumor, and its expression level was lowest in mucinous adenocarcinoma and poorly differentiated adenocarcinoma （both P〈0.05）. Conclusion： The miR-451 expression in CRC tissues is generally decreased, and the decreasing degree is related to the histologic classification and differentiation of the tumor.
出处
《中国普通外科杂志》
CAS
CSCD
北大核心
2013年第4期447-451,共5页
China Journal of General Surgery
基金
国家自然科学基金资助项目(81072034)
