摘要
目的:探索伏隔核(NAc)壳部脑深部电刺激术(DBS)对大鼠肥胖症的治疗作用,阐明DBS治疗肥胖症的可行性。方法:30只大鼠随机分为DBS组和对照组,每组15只。2组大鼠均给予高脂饲料喂养并分别通过立体定向给予电极固定装置植入、电极植入,DBS组大鼠给予高频电刺激7d,对照组不给予刺激,电极拔除后继续观察28d。实验全程(57d)每日监测大鼠体质量、摄食量和饮水量。综合比较2组大鼠体质量增长率、摄食量和饮水量变化。结果:NAc壳电刺激第5天开始DBS组大鼠体质量增长率低于对照组(P<0.05),至实验结束时DBS组大鼠体质量平均增长幅度为10.2%,对照组为35.7%。电刺激期间DBS组大鼠摄食量低于对照组(P<0.05),刺激结束后2组大鼠摄食量差异无统计学意义(P>0.05)。2组大鼠饮水量在整个实验过程中差异无统计学意义(P>0.05)。结论:NAc壳部可以作为DBS治疗大鼠肥胖症的理想靶点,一方面限制摄食行为,降低摄食量;另一方面可能通过其与能量平衡系统相互作用,增加大鼠能量消耗,减缓体质量增长。
Objective To explore the effect on obesity by deep brain stimulation（DBS） in the rat nucleus accumbens（NAc） shell,and to elucidate the feasibility of DBS for obesity treatment.Methods 30 rats were randomly divided into DBS group（n=15） and control group（n=15）.High fat feed was given from the beginning,and electrode fixing device and electrode were implanted stereotacticly.The rats in DBS group were given high frequency stimulation for 7 d,the rats in control group were not given stimulation.After electrode pulled-out,the rats in two groups were observed for another 28 d.In the whole experimental course（57 d）,the body weight,food intake,and water intake were monitored every day.A comprehensive comparison of weight gain rate,food intake and water intake was conducted.Results From the fifth day after NAc shell stimulation,the weight gain rates of the rats in DBS group were lower than those in control group（P0.05）.At the end of the experiment,the weight gain rate reached to 10.2% and 35.7% in DBS group and control group.In the stimulation period,a food intake reduction was observed in DBS group（P0.05）.After stimulation,the difference of food intake was no longer existed.The water intake during the whole experiment had no obvious changes in both groups（P0.05）.Conclusion NAc shell could be an ideal target for DBS in treatment of obesity in rats.On the one hand,it can limit feeding behavior,and reduce food intake;on the other hand,it may interact with energy homeostasis system,increase energy expenditure and decrease weight growth of rats.
出处
《吉林大学学报（医学版）》
CAS
CSCD
北大核心
2013年第2期209-212,共4页
Journal of Jilin University：Medicine Edition
基金
国家自然科学基金资助课题(30973085)
关键词
肥胖症
脑深部电刺激
伏隔核
obesity
deep brain stimulation
nucleus accumbens
