摘要
目的:探讨人端粒酶基因(hTERC)与人乳头瘤病毒L1壳蛋白(HPV L1)检测对子宫颈病变诊断及进展风险评估以及指导临床最佳的分流管理与治疗。方法:搜集因宫颈疾病就诊的75例患者临床资料,根据组织病理学结果将其分为5组,其中正常/炎症18例、CINⅠ19例、CINⅡ16例、CINⅢ14例、宫颈鳞状细胞癌(SCC)8例。应用荧光原位杂交(FISH)方法检测hTERC基因在正常/炎症、CINⅠ、CINⅡ、CINⅢ及SCC组织标本中的表达情况和应用免疫组织化学方法检测HPVL1壳蛋白在正常/炎症、CINⅠ、CINⅡ、CINⅢ及SCC组织标本中的表达情况。结果:①hTERC基因阳性表达率在正常/炎症、CINⅠ、CINⅡ、CINⅢ及SCC组中分别为5.56%、21.05%、50.00%、85.71%、100.00%,hTERC基因阳性扩增率随着宫颈组织病理学级别的升高逐渐呈现上升趋势(P<0.01)。②HPV L1壳蛋白在正常/炎症、CINⅠ、CINⅡ、CINⅢ和SCC中的阳性表达率分别为77.78%、68.42%、18.75%、7.14%、0.00%,HPV L1壳蛋白阳性率随着宫颈病变级别的加重呈现下降趋势(P<0.01)。③hTERC基因和HPVL1壳蛋白的4种表达类型以hTERC(-)/HPVL1(+)、hTERC(-)/HPVL1(-)、hTERC(+)/HPVL1(+)、hTERC(+)/HPVL1(-)的组合排序在宫颈病变中随着组织病理学级别增高而呈现上升的趋势(P<0.01)。结论:hTERC基因与HPV L1壳蛋白检测可以作为子宫颈病变早期诊断及进展风险评估的有效指标。
Objective： To explore the value of human telomerase RNA component gene （hTEIIC） and human paplllomavlrus LI protein （ HPV L1 ） detection for diagnosis and risk assessment of progress of cervical lesions, and direct optimal clinical triage management and treatment. Methods： The clinical data of 75 patients who went to the hospital because of cervical diseases were collected, then the pa- tients were divided into five groups according to histopathological results： normal/inflammation group （18 patients） , CIN I group （19 pa- tients）, CIN II group （16 patients）, CIN III group （14 patients）, and SCC group （8 patients） . The expression rates of hTERC gene in the five groups were detected by FISH, while the expression rates of HPV L1 capsid protein in the five groups were detected by immunohisto- chemical method, Results： The positive expression rates of hTERC gene in normal/inflammation group, CIN I group, CIN II group, CIN III group, and SCC group were 5. 56%, 21.05%, 50. 00%, 85.71%, and 100. 00%, respectively, which showed increasing trends with the increase of pathological grades of cervical lesions （ P 〈0.01 ） . The positive expression rates of HPV L1 capsid protein in normal/inflamma- tion group, CIN I group, CIN II group, CIN III group, and SCC group were 77.78%, 68.42%, 18.75%, 7.14%, and 0. 00%, respec- tively, which showed decreasing trends with the increase of pathological grades of cervical lesions （P 〈 0. 01 ） . Four types of expressions of hTERCgeneandHPVL1 protein werehTERC （-） /HPVL1 （＋）, hTERC （-） /HPVL1 （-）, hTERC （＋） /HPVL1 （＋）, and hTERC （ ＋ ） /HPVL1 （ - ）, respectively, which showed increasing trends with the increase of pathological grades of cervical lesions （P 〈 0. 01 ）. Conclusion： The detection of hTERC gene combined with HPV L1 capsid protein can be used as an effective indicator for early diagnosis and risk assessment of progress of cervical lesions.
出处
《中国妇幼保健》
CAS
北大核心
2013年第11期1714-1717,共4页
Maternal and Child Health Care of China
基金
河南省卫生厅科技人才创新工程资助项目〔201004122〕
