摘要
根据反应机理和产物的化学结构 ,建立了嗜热脂肪芽孢来源的β 半乳糖苷酶催化乳糖水解和转半乳糖苷反应偶合的动力学模型 ,模型中包含了低聚三糖和低聚四糖的生成。通过优化计算 ,估算反应动力学参数 ,结果表明该模型能较好地与实验结果相吻合。
In the course of lactose hydrolysis,the β galactosidase produced variable amounts of galacto oligosaccharide(GOS)due to the transgalactosydic reaction.According to enzymatic reaction mechanism by β galactosidase and structures of GOS,a model was developed to describe the process including GOS(trisaccharide and tetrasaccharide)formation by Bacillus stearothermophilus β galactosidase.The Simple optimization method was employed to determine the kinetic parameters of rate expression.The kinetic model showed a good agreement between experimental and simulated results.
出处
《生物工程学报》
CAS
CSCD
北大核心
2000年第6期735-739,共5页
Chinese Journal of Biotechnology
