摘要
目的:研究大肠癌发生发展中的腺瘤→癌模式理论。方法:外科手术切除的大肠腺癌30例、腺瘤11例、癌自身正常粘膜16例。以大肠肠管长轴为纵轴在横、纵、水平三个相互垂直的方向上对以上三种组织取材和切片,随机确定视野,按照体视学原理随机测试以上三种组织,根据有关体视学公式计算出体视学参数(Vvg、Svg、Rsvg、Lg、Vvn、Svn、Rsvn、Rnp、入、v、Nv、Kv、k、s),比较这些参数在不同组织间的差异。结果:参数Vvn、Svn、Rnp、v、s和Vvg的大小是依大肠正常组织、腺瘤、腺癌的捧序而递增,Rsvn、久、Nv、k和Rsvg的参数大小则依正常粘膜、腺瘤及癌排序方向而递减,且三种组织间上述参数的比较差异均有显著性;结果表明三方向全取的取材方法在描述组织间的差异方面优于单方向取材方法。结论:本文对大肠癌组织和细胞体视学参数的定量分析结果,进一步支持大肠癌发生、发展学说中的“腺瘤→癌”模式理论;三方向全取的取材方法有进一步提高其诊断的效果。
Objective: To study the model from adenoma to carcinoma in colorecta carcinoma. Methods: The mass specimens of 30 colorectal carcinomas, 11 colorectal adenomas and 16 normal colorectal musocas were obtained from the undergoing surgical re-section. The three tissues were cut in three mutually vertical directions, namely: transversely, longitudinally and horizontally with the long axis of colorectal canal as the longitudinal axis. Measurement were made according to the sterelogical principle in the random-ly chosen fields of the slices cut from the mass specimens of colorectal carcinoma, adenoma and normal colorectal tissue in three differ-ent directions and the sterological parameters were acquired according to stereological formulae, The parameters were compared to an-alyze the differences among their tissues. Results: Most of the stereological parameters showed a continuously processing variation with the heteromorphism of the glands and cells, The value of the stereological parameters of Vvn、Svm、Rnp、v、s and Vvg were gradually increased but those of Rsvn、λ、Nv、k and Rsvg were graually decreased in terms of the sequence of colorectal carcinoma, adenoma and normal tissue. In addition, there were significant differences among the parameters in the three kinds of tissues. The signifacnt differ-ences was higher with the method of sampling in three directions than that in one direction. Conclusion: The model of occurrence and development that colorectal carcinoma occurs from adenoma to carcinoma was further supported by the stereological quantification analyses of on the tissues and cells of colorectal carcinoma in our study. the methods of sampling in all of the three directions have higher value to elevate the effects of determination and diagnosis with stereological parameters.
出处
《临床肿瘤学杂志》
CAS
2000年第1期5-9,共5页
Chinese Clinical Oncology
基金
国家自然科学基金资助项目(课题号:39000029)
关键词
大肠腺癌
腺瘤
方向性
体视学
Colorectal Carcinoma Colorectal adenoma Direction Stereology
