摘要
建立了雷公藤药材中雷公藤甲素的含量测定方法。采用Gemini-C18(250 mm×4.60 mm,5μm)色谱柱;流动相:乙腈-水(25∶75);检测波长218 nm;流速1.0 mL.min-1,柱温为30℃。结果表明:雷公藤甲素在5.82～58.20μg.mL-1浓度范围内与峰面积呈良好的线性关系(r=0.9996),平均回收率为101.49%,RSD=2.02%。本方法简便、准确、重现性好,可用于雷公藤药材中雷公藤甲素的含量测定。
A HPLC method was established for determination of triptolide in Tripterygium medicinal materials. The Gemini - C18 （250 mm × 4.60 mm, 5 μm） column was used. The mobile phases consisted of acetonitrile and water （25 ： 75 ）, the flow rate was 1.0 mL min- 1 and the detection wavelength was 218 nm. The linear concentration range of Diphen- hydramine hydrochloride was at 5.82 - 58.20 μg mL-1 （r=0. 9996）. The average recovery was 101.49% and RSD was 2.02%. The method was simple, reliable, accurate and can be used in the quality control of triptolide in Tripterygium medicinal materials.
出处
《广州化工》
CAS
2013年第8期132-133,218,共3页
GuangZhou Chemical Industry
