摘要
目的建立铁皮枫斗HPLC特征图谱。方法采用ZorbaxSBC18色谱柱；流动相为乙腈．0．2％的甲酸溶液，梯度洗脱；检测波长为270nm；柱温为35℃；流速为1．0mL·min^-1。结果铁皮枫斗标示出36个特征共有峰，10批样品的相似度为0．898～0．969。结论本方法准确可靠，重复性好，为铁皮枫斗的质量控制提供了一定的方法依据。
OBJECTIVE To establish the method of characteristic spectrum analysis on Dendrobium officinale of Fengdous by HPLC. METHODS HPLC was used with the Zorbax SB C18 column, the acetonitrile-0.2%methanoic acid solution with gradient elution was employed as a mobile phase, detection wavelength was 270 nm, column temperature was 35℃, and flow rate was 1.0 mL.min-l. RESULTS Thirty six common peaks were separated in Dendrobium officinale of Fengdous, the similarities of 10 batches were 0.898-0.969. CONCLUSION The method is accurate and reliable, has a better reproducibility, and provides a reference for the quality control ofDendrobium officinale of Fengdous.
出处
《中国现代应用药学》
CAS
CSCD
2013年第4期390-394,共5页
Chinese Journal of Modern Applied Pharmacy
基金
广东省产学研合作项目(2009B090300333)
广东省产学研结合项目(2011B090400319)
关键词
铁皮枫斗
特征图谱
高效液相色谱法
Dendrobium officinale species of Fengdous
characteristic spectrum
HPLC
