摘要
采用氨法破壁提取RNA ,当酵母质量分数为 10 % ,氨为 1 0 %时 ,采用分步沉淀法 ,调蛋白质的等电点后 ,再加入复合蛋白质沉淀剂去除蛋白质 ,可获得纯度在 83%的优质酵母RNA ,核酸的提取率是干酵母的2 %以上。
The cell wall of beer yeast was broken with ammonia method The best yeast and ammonia concentration for breaking cell was 10% and 1 0% respectively The protein was removed by regulating the isoelectric point and chemical agents Then the high quality yeast RNA with the purity of 83% was obtained
出处
《药物生物技术》
CAS
CSCD
2000年第1期35-37,共3页
Pharmaceutical Biotechnology
基金
福建省教委基金!J960 14
