摘要
目的探讨肝细胞生长因子（hepatocytegrowthfactor，HGF）对创伤性关节软骨损伤的修复作用。方法选用大耳白兔25只，随机分成5组，每组5只。在两侧髌股关节股骨侧的关节面中心分别造成直径3.5mm的全层关节软骨缺损。选择右侧为实验组，自术后起，膝关节内注射10μg／ml的HGF0．1ml，每周3次，共4周;左侧为对照组，与实验组同一时间注射等量生理盐水。于术后4、6、8、12、24周分别取材进行大体、光镜和透射电镜观察。结果HGF实验组修复组织填充快，质量好。4周时缺损区充填完全，再生软骨与纤维组织比例约1：1。24周时再生组织与周围正常软骨外观相近，肉眼难以区分；光镜下损伤区基本恢复正常软骨组织结构；电镜下可见到多数形态成熟的透明软骨细胞。而盐水对照组6周时缺损区仍有浅表凹陷，并以纤维组织性修复为主。除术后4周外，其余各期两组的组织学评分差异均有显著性意义（P＜0.01）。实验同时发现再生软骨干24周后变薄，个别标本有裂隙形成。结论HGF能有效地促进创伤性关节软骨缺损的修复，为临床无创性修复关节软骨缺损提供了一条新途径。
Objective To investigate the mechanism of hepatocyte growth factor(HGF) in articular cartilage defect repair in vivo. Methods Cylindrical, full-thick articular cartilage defects, 3. 5 mm in diameter, were produced in the knee joints of 25 rabbits. 1μg HGF dissolved in 0.1 ml saline was injected into the right knee joints 3 times a week for 4 weeks and 0. 1 ml normal saline was injected into the left knee joints as a control. The animals w ere killed respectively in 4, 6, 8, 12, 24 weeks, and the macroscopic, histologic and ultrastructural examinations were performed. Results The repairing process in experimental group was faster and hetter than that in the control. The histological and ultrastructural examination showed features of hyaline cartilage, whereas fibrous tissue was seen in the control group. The data of histological grades showed significant statistical difference (P < 0.01 ). Conclusion HGF plays an important role in cartilage metabolism and it can effectively facilitate the repair of articular cartilage defect, and provides a new non-traumatic method for the treatment of articular cartilage defects.
出处
《中华骨科杂志》
CAS
CSCD
北大核心
2000年第3期181-184,共4页
Chinese Journal of Orthopaedics
关键词
肝细胞生长因子
组织学
关节软骨缺损
修复
Hepatocyte growth factor
Cartilage, articular
Histology, comparative
