摘要
孤儿基因为某物种基因组内特有的,在其它物种内没有同源基因存在的特殊基因。对物种孤儿基因的鉴定和分析已成为比较基因组学研究的一个新兴领域。本研究通过生物信息学的分析,着重研究了49个葡萄孤儿基因在各发育阶段和各器官中的特异表达特性、亚细胞定位预测和共表达基因群的生物学过程基因本体论(GO)分析。结果显示,35个基因在NCBI的EST数据库中存在EST表达证据,其中有7个基因具有器官表达特异性,且大部分在生殖器官中特异表达。通过亚细胞定位预测到大部分葡萄孤儿基因为分泌蛋白。GO分析表明葡萄孤儿基因的共表达基因群主要参与寡肽转运和跨膜运输等生物学过程,这与葡萄孤儿基因的亚细胞定位预测结果相一致。本文研究结果将为葡萄孤儿基因的进一步功能分析提供重要研究基础。
Orphan gene is a type of particular gene, which is unique in the genome of a certain species or has no known homologs in other species. The identification and characterization of orphan genes of a certain species has been an emerging field of comparative genomics. Using the approaches of bioinformatics, we mainly survey the development or organ specific expression, subcellular localization prediction and Gene Ontology （GO） analysis of 49 orphan genes in grape. We found that 35 grape orphan genes （GOGs） have EST evidence in NCBI EST database, and seven of them are organ specific expressed. Most of the organ-specific orphan genes are expressed after sexual maturation. Subcellular localization prediction revealed that the GOGs were significantly enriched in proteins targeted to the secretory pathway. GO analysis indicated the significant enrichment of the co-expressed genes of orphan genes in oligo-peptide transport and transmembrane transport functional categories, which are consistent w ith the subcellular localization prediction of GOGs. The results of this paper could provide a significant foundation for further research on the functional analysis of GOGs.
出处
《基因组学与应用生物学》
CAS
CSCD
北大核心
2013年第2期240-245,共6页
Genomics and Applied Biology
基金
农业部"948"项目(2011-G21)资助
