摘要
目的制备脑源性神经营养因子(brain-derived neurotrophic factor,BDNF)复合导管并对其性能进行评价。方法采用聚乳酸(PLA)作为支架材料,利用溶剂挥发法制备BDNF复合导管并对其浸入溶液前后的形态进行观察,利用万能材料实验机研究其力学性能,接触角测试仪检测其亲疏水性,ELISA测定释放液中BDNF的浓度并评价其体外释药特性。结果与结论 BDNF导管的拉伸强度为1.80 MPa,断裂伸长率为204.17%,接触角为65.4°,药物缓慢释放可达90 d,累积释放率约70%。表明制备的BDNF复合导管具有一定的柔韧性、亲疏水性和缓释特性。
Objective To prepare the brain-derived neurotrophic factor（BDNF） composite comluil and evaluate its properties. Methods A composite conduit made of polylactic acid was prepared by solvent evaporation method. The surface morphology was observed before and after drug release in phosphate buffer. The mechanical property was tested by universal material testing machine. The hydrophilic-hydrophobic properties were studied with contact angle instrument and drug release characteristics in vitro were evaluated by the determination of BDNF in release buffer with ELISA. Results and Conclusion The tensile strength of BDNF conduit was 1.80 MPa, the elongation at break was 204.17% ,and the contact angle was 65.4°. Drug release from the conduit could last 90 d, and the cumulative release rate was nearly 70%. The experimental results showed that the BDNF composite conduit has proper mechanical and hydrophilic-hydrophobic properties with an acceptable sustained-release profile.
出处
《军事医学》
CAS
CSCD
北大核心
2013年第3期207-210,共4页
Military Medical Sciences
关键词
脑源性神经营养因子
支架
导管
聚乳酸
brain-derived nerve neurotrophic factor
stents
conduit
polylactic acid
