摘要
目的:探讨EV71相关手足口病患儿外周血T细胞及细胞因子的水平及其意义。方法:将90例EV71感染手足口病患儿分为脑炎并发肺水肿组8例,单纯脑炎组30例,无并发症组52例。采用间接免疫荧光法检测外周血CD4+T及CD8+T细胞百分比,用双抗体夹心ABC-ELISA法检测血清IL-6、IL-10、TNF-α及IFN-γ含量。结果:并发症组CD4+及CD8+T淋巴细胞亚群的百分比相对无并发症组的降低,差异有统计学意义(F=9.304,P<0.05;X2=26.075,P<0.05),并发症组血清IL-10、IFN-γ和IL-6含量较无并发症组增高,差异有统计学意义(F=4.232,P<0.05;X2=16.975,P<0.05;X2=15.562,P<0.05),并发症组TNF-α含量和无并发症组差异无统计学意义。结论:外周血T细胞及细胞因子均参与了EV71相关手足口病重症并发症的发生过程,在手足口病的临床诊疗中可予以参考,早期鉴别出现重症并发症的病例,及时给与相应的治疗措施,降低手足口病的病死率和致残率。
Objective： To investigate the levels and significance of T cells and cytokines in children with enterovirus-71-related hand, foot and mouth disease （HFMD）. Methods： Children who were confirmed infected by enterovirus-71 were divided into three groups： 8 patients with pulmonary edema and cephalitis, 30 patients with cephalitis without pulmonary edema and 52 patients without complications. Serum IL-6, IL-10, TNF-et and IFN-γwere detected by ELISA. Indirect immunofluorescence was used to analyze the number ofCD4^＋ and CD8＋ T cells in leukomonocyte suspension. Results： The numbers ofCD4^＋ and CD8^＋ T cells in patients with complications were both smaller than that in patients without complications （F=9.304, P〈0.05; X^226.075, P〈0.05）. Compared with that in patients without complications, the levels of serum 1L-10, IL-6 and IFN-γ were increased in patients with complications （F=4.232, P〈0.05; X^2=-16.975, P〈0.05; X^2=- 15.562, P〈0.05）; At the same time, serum TNF-α level had no significant difference in the three groups. Conclusion： T cells and cytokines in peripheral blood may play an important role in the developmenf of enterovirus-71-related HFMD： It requires our attention to distinguish patients with complications and treat them as soon as possible, so as to cut down fatality and disability of HFMD.
出处
《现代生物医学进展》
CAS
2013年第7期1330-1332,共3页
Progress in Modern Biomedicine
关键词
手、足口病
T细胞
细胞因子
Hand, Foot and mouth disease （HFMD）
T cell
Cytokine
