摘要
为探明控制粳稻异交相关性状及其中亲优势的基因作用类型,利用秀堡RIL群体及其2个回交(BCF1)群体对穗抽出度、剑叶长、剑叶角度、穗剑高度差、倒2叶长、倒2叶角度、穗与倒2叶(穗二)高度差和倒1节间长8个异交相关性状及其中亲优势进行QTL定位。3个群体中共检测到45个显著的主效QTL(M-QTL),单个M-QTL的贡献率变幅为1.5%～80.3%。73.3%的M-QTL表现为加性效应,4.5%的M-QTL表现为部分或完全显性效应,22.2%的M-QTL表现为超显性效应。3个群体中共检测到82对显著的双基因上位性QTL(E-QTL)。RIL群体中检测到43对E-QTL,单对E-QTL的贡献率变幅为1.0%～7.0%,平均2.7%。在以秀水79为父本、与秀堡RIL群体回交的后代(XSBCF1群体)中检测到16对E-QTL,其中利用BCF1表型值检测到11对E-QTL,单对E-QTL的贡献率变幅为11.2%～36.8%,平均21.0%;利用中亲优势值检测到6对E-QTL,单对E-QTL的贡献率变幅为33.1%～76.8%,平均55.0%。在以C堡为父本、与秀堡RIL群体回交的后代(CBBCF1群体)中检测到23对E-QTL,其中利用BCF1表型值检测到16对E-QTL,单对E-QTL的贡献率变幅为6.2%～60.0%,平均24.0%;利用中亲优势值检测到7对E-QTL,单对E-QTL的贡献率变幅为21.3%～44.4%,平均31.0%。上述结果表明,粳稻异交相关性状是由多位点控制的,基因对性状本身的作用类型以加性效应为主;粳稻异交相关性状中亲优势主要遗传基础为超显性效应和上位性效应。
To understand the types of gene action controlling outcrossrelated traits and its midparental heterosis, QTL mapping of eight outcrossingrelated traits including panicle exsertion degree （PED）, flag leaf length （FLL）, flag leaf angle （FLA）, vertical distance between panicle tip and flag leaf tip （DPFT）, second leaf length （SLL）, second leaf angle （SLA）, vertical distance between panicle tip and second leaf tip （DPST） and length of the first internode （LFI） from the top was conducted using 254 Xiushui 79/C Bao recombinant inbred lines （RILs） and two backcross hybrid populations derived from these RILs. 45 maineffect QTLs （MQTL） were identified in the three populations. The percentage of phenotypic variance explained by each QTL ranged from 1.5% to 80.3%. Among them, 73.3% QTL showed an additive effect, 4.5% a partialtocomplete dominant effect, and 22.2% an overdominant effect. Eightytwo pairs of digenic epistatic QTL （EQTL） were detected in the 3 populations,of which 43 pairs were detected in RIL population, and the percentage of phenotypic variance explained by each pair of QTL ranged from 1.0% to 7.0%, with an average 2.7%. Sixteen pairs of EQTL were detected in XSBCF1 population. Among them, 11 pairs were detected by using XSBCF1 phenotypic value, and the percentage of phenotypic variance explained by each EQTL ranged from 11.2% to 36.8%, with an average 21.0%. Six pairs of EQTL were detected by using midparental heterosis value （HMP）, and the percentage of phenotypic variance explained by each EQTL ranged from 33.1% to 76.8%, with an average 55.0%. Twentythree pairs of EQTL were detected in CBBCF1 population. Among them, 16 pairs of EQTL were detected by using BCF1 phenotypic value, and the percentage of phenotypic variance explained by each pair of EQTL ranged from 6.2% to 60.0%, with an average 24.0%. Seven pairs of EQTL were detected by using the midparental heterosis value, and the percentage of phenotypic variance explained by each pair of EQTL ranged from 21.3% to 44.4%, with an average 31.0%. These results showed that outcrossingrelated traits themselves were mainly controlled by multiple loci with additive effects, and the primary genetic basis of midparent heterosis for outcrossingrelated traits was overdominant and epistatic effect in japonica rice.
出处
《中国水稻科学》
CAS
CSCD
北大核心
2013年第3期247-258,共12页
Chinese Journal of Rice Science
基金
国家863计划资助项目(2010AA101301)
关键词
粳稻
异交性状
主效QTL
上位性QTL
japonica rice outcross-related trait main-effect QTL epistatic QTL
