摘要
Dabb类蛋白是2000年被发现的一类新蛋白家族,具体功能特征尚不明确。以耐盐碱能力极强的东北野生大豆G07256为试材,根据前期得到的野生大豆碱胁迫基因芯片表达谱,从中筛选出一个碱胁迫处理下显著上调表达的Dabb类基因(probe set为Gma.16010.1.S1_at),通过电子克隆和RT-PCR获得该基因全长cDNA序列,命名为GsDabb1。序列分析表明,该基因在多个植物物种中均有同源基因,但功能尚不明确。通过实时荧光定量PCR(Real-time quantitative PCR)分析该基因在野生大豆高盐、低温、干旱胁迫下的表达模式,表明该基因能够响应多种非生物胁迫。为研究该基因在胁迫过程中的功能,将全长基因转化模式植物拟南芥,对转基因植株的表型分析结果显示,转基因拟南芥的耐旱性得到增强,表明该基因参与植物的耐旱过程,并能够提高植物耐旱性。研究得到对植物干旱胁迫抗性起重要作用的关键基因,为作物抗逆分子育种提供基因资源和奠定理论基础。
Dabb, a new protein family, was discovered in 2000, whose specific function is unclear for now. In this study, a up-regulated gene （probe set as Gma.16010.1.S1 at） was selected, based on the wild soybean gene chip expression profiling under alkaline stress. Full-length cDNA sequence of the gene was obtained by silico cloning and RT-PCR, named as GsDabbl. Sequence analysis indicated that the gene had homologous genes in a few plant species, but the gene function was unclear. Real-time quantitative PCR was used to analyze the expression pattern of the gene was induced by high salinity, low temperrature, drought stresses. To study the gene function in osmotic stress, the gene was thansformated into Arabidopsis plants. The results indicated that heterologous expression GsDabbl genecould improve plant tolerance to drought stress. This study will enrich gene resources and theoretical basis for plant genetic engineering.
出处
《东北农业大学学报》
CAS
CSCD
北大核心
2013年第4期1-7,F0002,共8页
Journal of Northeast Agricultural University
基金
黑龙江省高校科技创新团队建设项目(2011TD005)
国家自然科学基金项目(31171578)
国家转基因育种重大专项(2011ZX08004-002)
