摘要
目的:研究伸筋活血合剂对动物急性炎症、疼痛和软组织损伤模型的作用。方法:取ICR小鼠和SD大鼠随机分为模型组,阳性药吲哚美辛组,伸筋活血合剂低、中、高剂量组。灌胃给药,连续7 d,小鼠和大鼠剂量分别为6,18,54 g.kg-1和2,6,18 g.kg-1,吲哚美辛分别为10,5 mg.kg-1。通过小鼠耳廓肿胀法、大鼠足跖肿胀法、小鼠腹腔毛细血管通透性法观察其抗炎作用;采用小鼠醋酸扭体法、热板法观察其镇痛作用;采用大鼠急性软组织损伤模型观察其对软组织损伤的修复作用。结果:伸筋活血合剂低、中、高剂量均可以降低二甲苯致小鼠耳肿胀率(P<0.05,P<0.01,P<0.01),高剂量组药效最佳;高剂量可以降低角叉菜胶致急性炎症的大鼠的足肿胀率(P<0.05);中、高剂量对醋酸致小鼠腹腔毛细血管通透性提高有显著抑制作用(均P<0.01);中、高剂量对醋酸所致的小鼠扭体疼痛有一定的抑制作用(均P<0.05);对提高小鼠耐热痛能力无明显作用;低、中、高剂量能显著降低损伤大鼠的外观损伤积分(P<0.05,P<0.05,P<0.01),改善损伤组织的病理学形态。结论:伸筋活血合剂在抗急性炎症、镇痛、急性软组织损伤的修复方面显示出了不同程度的药效学作用。
Objective： To study the anti-inflammatory, analgesic function and therapeutic effect on acute soft tissue injury of Shenjin Huoxue mixture（SJHXM）. Method： ICR mice and SD rats were divided into 5 groups randomly including model control group, indomethacin group and SJHXM groups. SJHXM was given at dose of 6, 18, 54 gkg-1 in mice and 2,6,18 gkg-1 in rats for 7 days while indomethacin was 10, 5 mgkg-1, respectively. The anti-inflammatory effects of SJHXM were observed with the models of mouse ear swelling induced by xylene, rat paw swelling induced by carrageen, and mouse increasing peritoneal capillary permeability induced by acetic acid. Methods of acetic acid-induced writhing and hot-plate test in mice were used to study the analgesic effect. Models of acute soft tissue injury in rats caused by strike were established to observe the therapeutic effect of SJHXM. Result： Compared with the model group, SJHXM could significantly inhibit the ear swelling, lessen the paw swelling in rats, decrease the capillary permeability and the writhing times of mice, improve tissue repair ability, yet the pain threshold was almost unchanged. Conclusion： SJHXM showed anti-inflammatory,analgesic function and alleviated acute soft tissue injury to various degree.
出处
《中国实验方剂学杂志》
CAS
北大核心
2013年第10期269-272,共4页
Chinese Journal of Experimental Traditional Medical Formulae
基金
上海市卫生局2010-2011年度中医药科研基金[2010L066A]
关键词
伸筋活血合剂
药效
抗炎
镇痛
软组织损伤
Shenjin Huoxue mixture
pharmacodynamics
anti-inflammatory
analgesic
acute soft tissue injury
