摘要
目的:探讨补充"健脾"中药对急性运动大鼠肌肉、肝脏线粒体呼吸链复合体活性的影响。方法:27只SD大鼠,随机分成正常对照组(9只)、急性运动组(9只)、中药补剂组(9只)。采用双抗体两步夹心酶联免疫吸附法(ELISA)测定大鼠肌肉、肝脏线粒体呼吸链复合体活性浓度。结果:急性运动组大鼠肌肉、肝脏线粒体呼吸链复合体活性浓度略高于正常对照组,但无显著差异;而中药补剂组大鼠肌肉组织的呼吸链复合体Ⅰ、Ⅲ及肝脏组织的呼吸链复合体Ⅱ、Ⅲ活性明显高于正常对照组(P<0.05)。结论:"健脾"中药可提高大鼠肌肉、肝脏线粒体呼吸链酶复合体活性,维持线粒体正常功能,改善急性运动过程中的能量代谢。
Purpose ： the research is to investigate the effects of Chinese herbal medicine of ＂ strengthening spleen＂ on the activities of mitochondrial respiratory chain complexes from skeletal muscles and livers in acute exercise rats. Method： 27 SD rats were randomly divided into the normal control group （9 rats）, the acute exercise group （9 rats）, the Chinese herbal medicine group （9 rats）. The activities of mitochondrial respiratory chain complexes from skeletal muscles and livers were detected by the method of enzyme linked immunosorbent assay （ELISA）. Result： there are no significant differences in the activities of mitochondrial respiratory chain complexes from skeletal muscles and livers of rats between the acute exercise group and the normal control group. The activities of mitochondrial respiratory chain complexes from skeletal muscles and livers of rats in Chinese herbal medicine group are increased markedly compared with the normal control group . Conclusion： The activities of mitochondrial respiratory chain complexes from skeletal muscles and livers of rats could be increased markedly through the Chinese herbal medicine of ＂strengthening spleen＂. The Chinese herbal medicine of ＂strengthening spleen＂ could maintain the normal functions of mitochondria and improvethe energy metabolism in the state of acute exercise.
出处
《成都体育学院学报》
CSSCI
北大核心
2013年第5期91-94,共4页
Journal of Chengdu Sport University
基金
广东省体育局科技攻关与服务项目:"运动疲劳状态下线粒体呼吸链复合体酶的动态变化及中药补剂的干预"(2012ONS053)
关键词
呼吸链复合体
急性运动
“健脾”中药
mitochondrial respiratory chain complexes
acute exercise
Chinese herbal medicine of ＂strengthening spleen＂
