摘要
目的构建紫苏属种、变种之间系统发育关系,准确鉴别紫苏Perilla frutescens var.arguta、白苏P.frutescens与其他变种。方法提取紫苏、白苏及其他变种的DNA,对ITS和psbA-trnH序列扩增和测序,计算物种种内(变种内)种间(变种间)Kimura 2-parameter(K2P)遗传距离。采用最简约法(MP)和邻接法(NJ)构建分子系统树,进行系统发育和鉴定分析。结果 ITS和psbA-trnH MP系统树显示白苏和紫苏的不同地域的样本聚为一单系分支(支持率为100%和93%),紫苏与回回苏构成姐妹群分支(支持率为95%和90%),野生紫苏和耳齿紫苏构成一单系分支(支持率为100%和90%);白苏、紫苏与其他变种间的ITS和psbA-trnH序列遗传距离0.008 21～0.018 18和0.002 38～0.029 31,明显大于白苏与紫苏间遗传距离0～0.001 65和0,大于各变种内遗传距离。结论白苏和紫苏合并为一个种紫苏;紫苏与回回苏亲缘关系最近,野生紫苏与耳齿紫苏亲缘关系最近;ITS和psbA-trnH序列可以准确鉴别紫苏与其他变种。
Objective In order to investigate the phylogenetic relationship of Perilla frutescens and its varieties, and to identify P. frutescens and its varieties in Perilla L. Methods Total genomic DNA was isolated from P. frutescens and its varieties. Nuclear DNA ITS and chloroplast gene psbA-trnH sequence were amplified and sequenced. The inter- and intra-specific Kimura 2-Parameter （K2P） distances were calculated. Phylogenetic analyses including Maximum Parsimony （MP） and Neighbor-Joining （N J） of nuclear DNA （ITS） and chloroplast gene psbA-trnH sequences were conducted individually. Results The MP tree （ITS and psbA-trnH） indicated that the different geographical population of P.frutescens and P.frutescens var. arguta formed a monophyletic elade [bootstrap （BS） = 100% and 93%], which was a sister branch to P.frutescens var. crispa （BS = 95% and 90%）. Wild P.frutescens var. purpurascens and P.frutescens var. auriculato-dentata formed a monophyletic clade （BS = 100% and 90%）. The inter-specific genetic distances （ITS andpsbA-trnH） of P.frutescens, P.frutescens var. arguta, and other varieties were 0.008 21---0.018 18 and 0.002 38----0.029 31, which were obviously higher than those of P.frutescens and P.frutescens var. arguta at 0--0.001 65 and 0, and were higher than intra-specific genetic distances of each other variety. Conclusion P. frutescens and P. frutescens var. arguta should be classified into one species. P. frutescens is relatively closed to P. frutescens var. crispa. The closest relationship is found between wild P. frutescens var. purpurascens and P. frutescens var. auriculato-dentata. ITS andpsbA-trnH are two efficient barcodes for the identification of P.frutescens and its varieties.
出处
《中草药》
CAS
CSCD
北大核心
2013年第8期1027-1032,共6页
Chinese Traditional and Herbal Drugs
基金
科技部国家星火计划项目(2012GA750004)
国家自然科学基金面上项目(31070171
31270236)
关键词
紫苏属
紫苏
ITS
PSBA-TRNH
鉴定
Perilla L.
Perilla frutescens （Linn.） Britt.
IT S
psbA -trnH
authentication
