摘要
目的探讨辅助性T细胞17（Th17）相关因子IL-17A，IL-6与TNF-α在慢性HBV感染者发病过程中的作用。方法采用流式细胞小球微阵列术（cytometric bead array，CBA）检测37例慢性乙型肝炎（CHB组）、40例乙型肝炎肝硬化（LC组）、10例肝癌（HCC组）患者和13例健康体检者[正常对照（NC）组]血清中IL-17A，IL-6与TNF-α水平，并比较3种细胞因子水平在LC Child-pugh分级中的作用；分析3种细胞因子水平相互间的相关性。结果CHB组和LC组血清IL-17A，IL-6与TNF-α水平均明显高于NC组，HCC组血清IL-17A，IL-6水平均明显高于NC组。3种细胞因子水平均与LC Child-pugh分级密切相关。3种细胞因子水平相互间有显著相关性。结论3种细胞因子水平可能在CHB，LC患者病情的进展以及预后中起着重要作用。3种细胞因子均可作为LC程度的判断指标。
Objective To explore the role of helper T lymphocyte associated factors such as IL-17A, IL-6 and TNF-α in the pathogenesis of chronic HBV infection. Methods Specific cytokines of helper T lymphocyte such as IL-17A,IL-6 and TNF- α in serum of 37 patients with chronic hepatitis B （CHB）, 40 patients with chronic liver cirrhosis （LC）, 10 patients with hepatoeellular carcinoma （HCC） and 13 healthy examined people Eas normal controls （NC）] were measured by Cytometric Bead Array. The level of three cytokines were analysised and showed the relationship between the level and the class of child-pugh and the relationship of three factors. Results The concentration of IL-17A, IL-6 and TNF-α of patients with CHB and LC were higher than that of NC. The level of IL-17A, IL-6 of patients with hepatocellular carcinoma were higher that of normal control. The level of IL-17A,IL-6 and TNF-α was closely related to the class of child-pugh. Three cytokines had significant correlation. Conclusion The level of IL-17A, IL-6 and TNF-α may play the important role in the progress and prognosis of CHB and LC. The level of these three cytokines can be used as a marker to determine the degree of liver cir rhosis.
出处
《现代检验医学杂志》
CAS
2013年第2期74-76,共3页
Journal of Modern Laboratory Medicine
