摘要
目的:探究AnnexinA1对小细胞肺癌细胞生物学行为的影响。方法:构建干扰AnnexinA1的慢病毒载体,转染至SBC-5细胞株;体外增殖、迁移、侵袭实验观察下调AnnexinA1表达对小细胞肺癌细胞株生物学行为的影响。结果:干扰AnnexinA1的细胞株ANXA1 RNAi-SBC-5构建成功。与转染空载体的对照细胞相比,抑制AnnexinA1的表达能够显著抑制SBC-5细胞的增殖、迁移、侵袭能力。结论:AnnexinA1表达可能与肺癌骨转移密切相关。
Objective：To explore the effect of AnnexinA1 on the biological behavior of small cell lung cancer cell lines. Methods：The expression of AnnexinA1 was down- regulated by RNA inference （RNAi） in SBC -5 cells. MTl＇,in vitro scratch test ,transwe11 based methods were used to detect the effect of AnnexinA1 on the proliferation, migration and invasion ability of small cell lung cancer cells. Results：The cells down - regulated the expression of AnnexinA1 were constructed. Down regulation of AnnexinA1 can decrease the proliferation ,migration and invasion potential in SBC -5 cell lines. Conclusion：The expression of AnnexinA1 is closely related to bone metastasis.
出处
《现代肿瘤医学》
CAS
2013年第5期917-921,共5页
Journal of Modern Oncology
基金
国家自然科学基金资助项目(编号:81172001)
