摘要
目的比较人脐带Wharton'sJelly来源间充质干细胞(mesenchymal stem cells derived from Wharton's Jelly,WJ-MSCs)与骨髓来源间充质干细胞(mesenchymal stem cells derived from bone marrow,BM-MSCs)对心肌梗死(myocardial infarction,MI)的治疗效果。方法分离鉴定人WJ-MSCs和BM-MSCs;小鼠随机分为MI组、WJ-MSC组、BM-MSC组,每组10只,通过结扎冠状动脉左前降支造成心肌梗死模型,于心肌梗死3h后分别静脉注射磷酸盐缓冲液1×106WJ-MSCs和1×106BM-MSCs;另设假手术组(Sham组,n=8),分离但不结扎冠状动脉,术后3h静脉注射磷酸盐缓冲液。超声心动图检查评价小鼠心功能;天狼猩红染色评价心肌纤维化程度。结果与MI组相比,WJ-MSC组左室短轴缩短率升高,左室收缩末内径下降,肺重/体重比下降,心重/体重比下降,心肌间质胶原沉积减少,且差异均有统计学意义(P<0.05);WJ-MSC组上述疗效与BM-MSC组相比无统计学差异(P均>0.05)。结论静脉移植WJ-MSCs能够改善心肌梗死后心功能,抑制心脏重塑,其疗效与BM-MSCs相当。
Objective To compare the curative effect of transplantation of mesenchymal stem cells derived from bone marrow （ BM-MSCs ） or from human Wharton＇s Jelly （ WJ-MSCs ） in mice. Methods WJ-MSCs and BM-MSCs were isolated and identified. All mice were randomly divided into myocardial infarction （ MI ） group, WJ-MSC group and BM-MSC group （ each n=10 ） . MI was induced by ligating left coronary anterior descending branch, and after 3 hours WJ-MSC group and BM-MSC group were respectively given intravenous injection of phosphate buffered saline （ PBS ） of 1 x 106 WJ-MSCs and PBS of 1 x l06 BM-MSCs. A sham-operation group （ sham group, n=8 ） was established and not given isolation and ligation, and given intravenous injection of PBS after 3 hours. The cardiac function was examined and reviewed by using echoeardiogram, and degree of myocardial fibrosis was reviewed after Sirius red staining. Results Compared with MI group, left ventricular fraction shortening （ LVFS ） increased, left ventricular end systolic diameter （ LVESd ） decreased, ratio of lung weight to body weight decreased, ratio of heart weight to body weight decreased, and myocardial collagen deposition decreased in WJ-MSC group （ P〈0.05 ） . Conclusion The intravenous transplantation of WJ-MSCs can improve cardiac function and inhibit ventricular remodeling after MI, and its curative effect is similar to that of intravenous transplantation of BM-MSCs.
出处
《中国循证心血管医学杂志》
2013年第2期196-199,共4页
Chinese Journal of Evidence-Based Cardiovascular Medicine
关键词
间充质干细胞
心肌梗死
脐带
小鼠
Mesenchymal stem cells
Myocardial infarction
Umbilical cord
Mice
