Hyaluronidase Proof for Endothelial Glycocalyx as Partaker of Microcirculation Disturbances
Hyaluronidase Proof for Endothelial Glycocalyx as Partaker of Microcirculation Disturbances
摘要
Covalent modification of bovine testicular hyaluronidase with chondroitin sulphate led to changes in the pattern of glycation of native and modified enzyme in its reaction with neutral saccharides and N-acetylhexosamines. Thus, mono- and di-saccharides inactivated the native hyaluronidase to a greater extent than the chondroitin sulfate-modified enzyme. N-acetylhexosamine, on the opposite, inactivated the modified hyaluronidase to a greater extent than the native one. These properties made it possible to use native and modified hyaluronidase as an informative research system for in vivo measurement of the predominant type of saccharide agents in the circulation. The proposed approach was experimentally substantiated by obtained results of the study on these interactions of hyaluronidase derivatives with hyaluronan fragments and their mixture. In a model of post-ischemic perfusion of the rat limb, the effect of hyaluronidase derivatives and their components on restoration of the microcirculation were tracked using laser Doppler flowmetry. Native hyaluronidase accelerated the restoration of initial level of microcirculation, but modified enzyme was markedly inhibited by glycocalyx degradation products. N-acetylhexosamine was positioned at the reducing terminal of these products as a natural label for these glycocalyx fragments. These and other data obtained under various experimental conditions supported the participation of endothelial glycocalyx in microcirculation disturbances.
参考文献67
-
1D. Bruegger, M. Rehrn, M. Jacob, D. Chappell, M. Stoeckel huber, U. Welsch, et al., Exogenous nitric oxide requires an endothelial glycocalyx to prevent postischemic coronary vascular leak in guinea pig hearts, Crit. Care 12 (2008) 73-84.
-
2M. Rehm, D. Bruegger, F. Christ, P. Conzen, M. Thiel, M. Jacob, et al. Shedding of the endothelial glycocalyx in patients undergoing major vascular surgery with global and regional ischemia, Circulation 116 (2007) 1896-1906.
-
3AV. Maksimenko, AD. Turashev, No-reflow phenomenon and endothelial glycocalyx of microcirculation, Biochem. Res. International[Online early access]. DOl: 10.1155/2012/859231, http://www.hindawi.com (accessed Jan. 12,2012).
-
4S. Weinbaum, J.M. Tarbell, E.R. Damiano, The structure and function of the endothelial glycocalyx layer, Annu. Rev. Biomed. Eng. 9 (2007) 121-167.
-
5L.N. Broekhuizen, H.L. Mooij, 1.1. Kastelein, E.S. Stroes, H. Vink, M. Nieuwdorp, Endothelial glycocalyx as potential diagnostic and therapeutic target in cardiovascular disease, Curr. Opin. Lipidol. 20 (2009) 57-62.
-
6M. Nieuwdorp, M.C. Meuwese, H. Vink, J.B. Hoekstra, 1.1. Kastelein, E.S. Stroes, The endothelial glycocalyx: A potential barrier between health and vascular disease, Curro Opin. Lipidol. 16 (2005) 507-511.
-
7B.F. Becker, D. Chappell, D. Bruegger, T. Annecke, M. Jacob, Therapeutic strategies targeting the endothelial glycocalyx: Acute deficits, but great potential, Cardiovasc. Res. 87 (2010) 300-310.
-
8S. Reitsma, D.W. Slaaf, H. Vink, M.A. van Zandvoort, M.G. oude Egbrink, The endothelial glycocalyx: Composition, functions, and visualization, Pflugers Arch. 454 (2007) 345-359.
-
9AV. Maksimenko, Effects of glycosaminoglycans in vascular events, Chem-Pharm. J. 42 (10) (2008) 3-13. (in Russian with English abstract).
-
10AW. Mulivor, H.H. Lipowsky, Inflammation- and ischemia-induced shedding of venular glycocalyx, Am. J. Physiol. Heart Circ. Physiol. 286 (2004) 1672-1680.
-
1周炼.橙皮糖制作要点[J].杭州食品科技,2007(3):17-19.
-
2宁西.西瓜皮糖酱制作法[J].农村新技术,1997(6):35-36.
-
3沈彦昆.传统工艺生产脆皮糖[J].食品工业,1995,16(6):7-9.
-
4墨菲.斯佩塞一流酒厂向中国引进单一麦芽苏格兰威士忌[J].中国食品,2015,0(21):142-142.
-
5抽皮糖加工技法[J].农家致富顾问,2012(10):27-27.
-
6邱澄铨,雷良萍.柚皮糖中含铜量的测定[J].吉安师专学报,1989(5):6-9.
-
7刘素英.柑桔皮风味食品的开发[J].农牧产品开发,1995(8):9-10.
-
8基于糖类的药物先导化合物的发现和优化研究[J].制药原料及中间体,2011(12):39-40.
-
9健康食品超市[J].食品与生活,2006(8):48-48.
-
10李乐怡.诺金集团:药食同源 科技先导[J].创新时代,2013(4):53-53.
