摘要
目的 用半定量多重聚合酶链反应 (PCR)法探讨胃肠道恶性肿瘤P16基因纯合性缺失的意义。方法 收集 31例患者手术切除的胃肠道原发恶性肿瘤组织标本 ,其中胃癌 18份 ,结肠癌 13份。采用半定量多重PCR检测其P16基因的纯合缺失情况。结果 在 31份胃肠道原发恶性肿瘤组织标本中 ,9份为P16基因纯合缺失 ,6份胃癌的P16基因纯合缺失中 ,4份是低分化腺癌 ,2份是未分化癌 ;3份结肠癌P16基因纯合缺失者为低分化腺癌。结论 P16基因异常对阐明胃肠道恶性肿瘤的生物学行为有一定的意义。半定量多重PCR方法稳定、简便 ,值得推广。
Objective To investigate the homozygous deletions of the P16 gene in gastrointestinal carcinoma by a semiquantitative polymerase chain reaction assay. Methods A semiquantitative multiple polymerase chain reaction assay was used to detect the tissue samples from 31 gastrointestinal carcinomas, including gastric carcinoma (18) and colorectal carcinoma (13).Results The homozygous deletions of the P16 gene were observed in 9 of the 31 carcinomas, including gastric carcinoma (6) and colorectal carcinoma (3). Conclusions Semiquantitative polymerase chain reaction assay is stable, simple, and convenient to detecting the homozygous deletions of the P16 gene. The homozygous deletions of the P16 gene may play a crucial role in biological behavior gastrointestinal carcinomas.
出处
《中华检验医学杂志》
CAS
CSCD
2000年第5期280-282,共3页
Chinese Journal of Laboratory Medicine
基金
中山医科大学 2 11工程重点学科建设项目! (980 97)
关键词
胃肠肿瘤
聚合酶链反应
P16基因
纯合性缺失
Gastrointestinal neoplasms
Genes, structural, neoplasm
Polymerase chain reaction
