摘要
目的:建立白术药材(烘术)的HPLC指纹图谱的分析方法。方法:加甲醇超声处理制样,采用HPLC法,以依利特Sinochrom ODS-BP柱(4.6 mm×250 mm,5μm)为色谱柱,以流动相为乙腈(A)-水(B)进行梯度洗脱,流速1.0 mL/min,检测波长242 nm,柱温30℃。结果:建立了白术药材的HPLC指纹图谱,找出了8个共有峰,指认了3个共有峰。结论:该方法较为准确、可靠,适用于白术药材(烘术)的质量控制。
Objectve：To establish an HPLC fingerprint analysis method for Atractylodis Macrocephalae Rhizoma（warm dried）.Methods：Took methanol extract of Atractylodis Macrocephalae Rhizoma as sample by ultrasonic processing,HPLC analysis was carried out on Sinochrom ODS-BP（4.6 mm×250 mm,5 μm）chromatographic column with mobile phase of acetonitrile（A）-water（B）,gradient elution with a flow at 1.0 mL/min,ultraviolet detection wavelength at 242 nm and column temperature at 30 ℃.Results：An HPLC fingerprint analysis method for Atractylodis Macrocephalae Rhizoma was developed.Eight common peaks were obtained and three peaks were identified.Conclusion：The method is accurate and credible which can be used for quality control of Atractylodis Macrocephalae Rhizoma（warm dried）.
出处
《中药材》
CAS
CSCD
北大核心
2013年第2期208-212,共5页
Journal of Chinese Medicinal Materials
基金
广东省科技创新平台建设专项(2011A091000005)
关键词
白术
烘术
白术内酯Ⅰ
白术内酯Ⅲ
苍术酮
HPLC指纹图谱
Atractylodis Macrocephalae Rhizoma
Warm dried
AtractylenolideⅠ
AtractylenolideⅢ
Atractrylone
HPLC fingerprint
