摘要
背景:实验拟通过土鳖虫对激素诱导骨髓间充质干细胞向脂肪细胞分化的影响,解释中药土鳖虫治疗股骨头缺血性坏死的作用机制。目的:观察土鳖虫对地塞米松诱导骨髓间充质干细胞成脂分化的干预作用。方法:通过大剂量地塞米松诱导体外培养的骨髓间充质干细胞成脂分化,在诱导成脂的同时给予土鳖虫含药血清干预。检测干预6d后细胞内成脂标志物三酰甘油、氧化物酶增殖物激活受体mRNA和脂肪酸相关蛋白mRNA的表达。结果与结论:土鳖虫可抑制骨髓间充质干细胞分化过程中氧化物酶增殖物激活受体基因和脂肪酸相关蛋白基因的表达,降低三酰甘油水平,说明土鳖虫药物可以逆转因大量激素导致的骨髓间充质干细胞成脂分化增加。
BACKGROUND： The experiments try to explain the pathogenesis of Eupolyphaga sinensis for the treatment of avascular necrosis of femoral head through investigating the effect of Eupolyphaga sinensis on the adipogenic differentiation of hormone-induced bone marrow mesenchymal stem cells.OBJECTIVE： To investigate the intervention effect of Eupolyphaga sinensis on the adipogenic differentiation of dexamethasone-induced bone marrow mesenchymal stem cells. METHODS： High-dose dexamethasone was used to induce the adipogenic differentiation of in vitro cultured bone marrow mesenchymal stem cells, and during the adipogenic differentiation, Eupolyphaga sinensis-containing serum was used for intervention. After 6 days intervention, expressions of adipogenic markers, such as triglyceride, peroxisome proliferator-activated receptor mRNA and adipocyte protein 2 mRNA, were detected. RESULTS AND CONCLUSION： Eupo/yphaga sinensis could inhibit the expression of peroxisome proliferator-activated receptor mRNA and adipocyte protein 2 mRNA in the differentiation of bone marrow mesenchymal stem cells, and could reduce the level of triglyceride, which suggested that Eupolyphaga sinensis drug can reverse the increased adipogenic differentiation of bone marrow mesenchymal stem ceils caused by high-dose hormones.
出处
《中国组织工程研究》
CAS
CSCD
2013年第14期2597-2602,共6页
Chinese Journal of Tissue Engineering Research
基金
福建省科技计划项目(2007F5060)
课题名称:土鳖虫对激素诱导骨髓间充质干细胞成脂分化的干预作用~~
关键词
干细胞
干细胞与中医药
骨髓间充质干细胞
股骨头坏死
成脂分化
中药
含药血清
土鳖虫
地塞米松
激素
省级基金
干细胞图片文章
stem cells
stem cells and traditional Chinese medicine
bone marrow mesenchymal stem cells
femoralhead necrosis
adipogenic differentiation
traditional Chinese medicine
drug-containing serum
Eupolyphaga sinensis
dexamethasone
hormone
provincial grants-supported paper
stem cell photographs-containing paper
