摘要
背景:理想的神经修复材料要有良好的生物相容性、生物可降解性、可塑性以及一定的机械强度。目的:观察自行研制的精氨酸-甘氨酸-天门冬氨酸多肽接枝聚(羟基乙酸-L-赖氨酸-乳酸)/聚乳酸/β-磷酸三钙/神经生长因子复合材料与大鼠骨髓间充质干细胞的细胞相容性。方法:将从SD大鼠骨髓中分离纯化的第3代骨髓间充质干细胞与精氨酸-甘氨酸-天门冬氨酸多肽接枝聚(羟基乙酸-L-赖氨酸-乳酸)/聚乳酸/β-磷酸三钙/神经生长因子复合材料或浸提液共同培养作为实验组,并以含体积分数10%胎牛血清的培养液培养骨髓间充质干细胞作为对照组。观察细胞在复合材料上的生长、存活及凋亡情况。结果与结论:MTT检测结果显示,共培养5,7d,实验组吸光度值高于对照组(P<0.05);流式细胞仪AnnexinV-FITC/PI双染法结果显示,实验组细胞凋亡率显著低于对照组(P<0.05);扫描电镜观察见骨髓间充质干细胞在精氨酸-甘氨酸-天门冬氨酸多肽接枝聚(羟基乙酸-L-赖氨酸-乳酸)/聚乳酸/β-磷酸三钙/神经生长因子复合材料表面生长良好,胞体发出多个突起,并交织成网状,呈典型的神经元样细胞表现。可见精氨酸-甘氨酸-天门冬氨酸多肽接枝聚(羟基乙酸-L-赖氨酸-乳酸)/聚乳酸/β-磷酸三钙/神经生长因子复合材料与骨髓间充质干细胞具有良好的细胞相容性,可作为优良的载体用于构建人工仿生神经。
BACKGROUND：Ideal nerve repair materials are characterized as good biocompatibility, biodegradability, plasticity, and certain mechanical strength. OBJECTIVE：To evaluate the cytocompatibility of rat bone marrow mesenchymal stem cel s with （glycolic acid-L-lysine-lactic acid）/poly lactic acid/β-tricalcium phosphate/nerve growth factor composites. METHODS：Passage 3 bone marrow mesenchymal stem cel s which were isolated from Sprague-Dawley rats were co-cultured with （glycolic acid-L-lysine-lactic acid）/poly lactic acid/β-tricalcium phosphate/nerve growth factor composite materials or their extracts, serving as experimental control. Another bone marrow mesenchymal stem cel s cultured in the culture medium containing 10%fetal bovine serum served as controls. Cel growth, survival and apoptosis on the composite materials were observed. RESULTS AND CONCLUSION：Results from 3-（4,5-dimethylthiazol-2-yl）-2,5-diphenyltetrazolium bromide assay showed that absorbance values in the experimental group were higher than those in the control group after co-culture for days 5 and 7 （P〈0.05）. Annexin V-FITC/PI double staining results showed that the apoptosis rate of the experimental group was significantly lower than that of the control group （P〈0.05）. Under a scanning electron microscope, bone marrow mesenchymal stem cel s grew wel on the surface of （glycolic acid-L-lysine-lactic acid）/poly lactic acid/β-tricalcium phosphate/nerve growth factor composite materials. Many protrusions ran out from the cel s, and they were woven into a mesh, which was typical performance of neuron-like cel s. These findings indicate that （glycolic acid-L-lysine-lactic acid）/poly lactic acid/β-tricalcium phosphate/nerve growth factor composite materials have good cytocompatibility with bone marrow mesenchymal stem cel s, which can be regarded as an excel ent carrier for artificial nerve construction.
出处
《中国组织工程研究》
CAS
CSCD
2013年第16期2913-2920,共8页
Chinese Journal of Tissue Engineering Research
基金
武汉市科技攻关计划项目(编号:200960323125)
课题名称:可吸收周围神经修复制品的应用研究与临床实验~~
