摘要
目的探讨IL-13基因Intron3＋1923多态性及血清IL-13和总IgE水平与儿童支气管哮喘（哮喘）的相关性。方法应用聚合酶链反应一限制性片段长度多态性（PCR—RFLP）方法对77例哮喘患儿（哮喘组）及50例健康儿童（健康对照组）行IL．13基因Intron3＋1923多态性分析，并采用ELISA法对哮喘患儿及健康儿童进行血清IL-13和总IgE水平的测定，并采用SPSS18．0软件对2组血清IL-13及总lgE水平进行分析。结果1．IL-13基因Intron3＋1923位点哮喘组与健康对照组之间的基因型及等位基因频率分布差异有统计学意义（基因型：X2=7．239，P〈0．05；等位基因频率：X^2=6．731，P〈0．01），等位基因T与哮喘关联。2．哮喘组患儿血清IgE水平为（50．98±27．97）mg／L，健康对照组为（42．85±27．19）mg／L，2组比较差异有统计学意义（t=3．067，P〈0．01）。3．哮喘组血清IL一13水平为（197．13±94．7）ng／L，健康对照组为（159．06±107．65）n∥L，2组比较差异有统计学意义（t=2．079，P〈0．05）。4．哮喘组IL-13基因Intron3＋1923不同基因型血清IgE水平的比较分别为CC：（30．25±19．75）me／L、CT：（57．50±27．64）mg／L、TT：（65．09±21．14）mg／L，组间比较差异有统计学意义（P〈0．05）；哮喘组IL．13基因Intron3＋1923不同基因型血清IL-13水平的比较分别为CC：（105．59±57．32）ng／L、CT：（222．45±74．00）ng／L、TT：（274．38±86．12）ng／L，组间比较差异也有统计学意义（P〈0．05）。结论IL-13基因Intron3＋1923多态性与儿童哮喘相关，其中T等位基因与儿童哮喘相关联。等位基因IL-13Intron3＋1923T影响血清IL-13、IgE水平，使血清IL-13、IgE水平升高。血清IL-13及总IgE水平升高与儿童哮喘相关。
Objective To explore the correlation of the polymorphism of gene IL-13 Intron 3 ＋ 1923and IL-13 and total IgE with pediatric asthma. Methods Polymerase chain reaction-restriction fragment length polymorphisms was used to analyze the polymorphism of gene IL-13 lntron 3 ＋ 1923 in 50 normal children （ healthy control group） and 77 asthma children （asthma group） ; enzyme linked immunosorbent assay was used to measure serum 1L-13 and total IgE, and used the SPSS 18.0 software to analyze the serum IL-13 and total IgE in 2 groups. Results 1. The gene type and ＇allele gene frequency distribution of gene IL-13 site Intron 3 ＋ 1923 in asthma group and healthy control group had statistical significance （ genotype ： X2 = 7. 239, P 〈 0.05 ; allele frequency ：X^2 = 6.731, P 〈 0.01 ） , Allele T had a rela-tionship with asthma. （ 2 ） The serum IgE concentration in asthma group was （50.98± 27.97） rag/L, and in normal control group was （42.85±27.19） mg/L, contrast difference had statistical significant （ t = 3. 067, P 〈 0.01 ）. （ 3 ） The serum IgE in asthma group was （ 197.13±94.7 ） ng/L and in healthy control group was （ 159.06±107.65 ）ng/ L, contrast difference had statistical significant （ t=2. 079, P 〈 0.05 ）. （ 4 ） Different genotype of gene 1L-13 lntron 3 ＋ 1923 in asthma group had distinguishing level of lgE： CC was （30.25 ±19.75） mg/L,CT was （57.50±27.64） mg/ L,TT was （65.09±21.14） mg/L,group contrast had significant difference （P 〈0.05） ; Different genotype of gene IL-13 lntron 3±1923 in asthma group had distinguishing level of IL-13, CC：（ 105.59±57.32） rig/L, CT： （222.45±74.00） ng/L,TT： （274. 38±86.12） ng/L, group contrast had significant difference （P〈0.05）. Conclusions Pol- ymorphism of gene IL-13 lntron 3 ＋ 1923 is relevant with children asthma,allele T gene is the correlative gene of chil- dren asthma. Allele IL-13 lntron 3 ＋ 1923T has an influence on the level of IL-13, IgE, which makes them higher. In- creasing total IL-13 and lgE in serum has a relationship with children asthma.
出处
《中华实用儿科临床杂志》
CAS
CSCD
北大核心
2013年第9期682-685,共4页
Chinese Journal of Applied Clinical Pediatrics
基金
内蒙古包头市卫生基金项目（wsjj2011043）
