摘要
目的探讨Survivin和第10号染色体缺失的磷酸酶及张力蛋白同源物基因（PTEN）及其编码蛋白在原发性肝细胞癌组织中的表达及其与肿瘤侵袭、转移的相关性。方法采用逆转录．聚合酶链反应（RT-PCR）技术及免疫组织化学染色法检测32例肝癌组织和对应的癌旁组织中Survivin和PTEN基因mRNA及蛋白的表达，并分析两者与肝癌临床病理因素的关系。结果SurvivinmRNA在原发性肝细胞癌组织及癌旁组织中的表达率分别为68．75％和3．13％，差异有统计学意义（P〈0．05）。SurvivinmRNA及蛋白在低分化肝癌组织、肿瘤直径〉5cm和有癌栓形成者中均明显增高（均P〈0．05）。PTENmRNA及蛋白在癌组织中表达率为43．75％，低于癌旁组织的87．50％（P〈0．05），其在低分化、肿瘤直径〉5em及有癌栓形成患者肝癌组织中的表达率分别为27．80％、31．60％、28．60％，均明显低于对应组的64．30％、61．50％、55．60％（P〈0．05）；SurvivinmRNA及蛋白表达与PTENmRNA及蛋白表达呈负相关（P〈0．05）。结论Survivin和PTEN表达与原发性肝细胞癌浸润转移密切相关，且两者的表达呈负相关。
Objective To study the expression of survivin and phosphatase and tensin homolog de- leted on chromosome ten （PTEN） mRNA and protein and their correlations with clinicopatholocal factors of hepatocellular carcinoma （HCC）. Methods Reverse transcriptase-polymerase chain reaction （RT-PCR） and immunohistochemistry were used to detect the expression of Survivin and PTEN mRNA and protein in 32 cases of HCC and their adjacent noncancerous tissues. Results The expression rate of survivin mRNA in HCC tissues and their adjacent noncancerous tissues was 68.75% and 3.13% respectively, and that of PTEN was 43.75% and 87.50% respectively. The high expression of survivin and the low expression of PTEN were positively corelated with the pathological grading, diameter of caicinoma and vein micro-metas- tasis in HCC （ P 〈 0. 05）. The expression of survivin showed a negative corelation with PTEN expression in HCC （P 〈 0. 05）. Conclusion These data show a corelation between survivin and PTEN expression and invasion and metastasis in HCC.
出处
《中华实验外科杂志》
CAS
CSCD
北大核心
2013年第6期1283-1285,共3页
Chinese Journal of Experimental Surgery
