摘要
经ＳＤＳＰＡＧＥ 分析发现，空肠弯曲菌细胞紧张性肠毒素（Ｃｙｔｏｔｏｎｉｃ ｅｎｔｅｒｏｔｏｘｉｎ ，ＣＥ） 的硫酸胺盐析粗提物除有一条６８ｋＤ 的带外，还有一些未分开的小分子物质，而经神经节苷脂ＧＭ１ 亲和层析后仅有６８ＫＤ 的一条带，即表明６８ｋＤ 的蛋白质为ＣＥ 的主要成分。ＣＥ 不耐热、ｐＨ 依赖和对胰酶有抗性。５６ ℃和６０ ℃加热３０ｍｉｎ 、１００ ℃加热１５ｍｉｎ 即可完全失活。其活性在ｐＨ６－０ 时最高，在ｐＨ３－０ 和９－０ 时均可使其完全丧失活性。在４ ℃保存超过３ｄ 后，其活性迅速降低。抗ＬＴ 血清能完全抑制ＣＥ 的活性。
Precipitate of Campylobacter jejuni cytotonic enterotoxin(CE) performed in an 80% saturated solution of ammonium sulfateit indicated that there were some little molecular proteins except the 68 kD main band on sodium dodecyl sulfate\|polyacrylamide gel electroploresis(SDS\|PAGE),whereas the eluate from GM 1 ganglioside affinity column chromatography exhibited only one 68kD band on SDS\|PAGE.The results suggest that CE mainly be consisted of 68 kD protein.The toxin is heat\|labile,pH dependent and resistant to trypsin,It could be completely inactivated by heating at either 56℃and 60℃for 30min or 100℃for 15min.The activity was maximum at pH6.0 and was completely inactivate at pH3.0 and pH9.0,and rapidly reduced after storage over 3 d at 4℃.The anti\|LT serum could completely inhibited the activity of CE.
出处
《微生物学报》
CAS
CSCD
北大核心
2000年第1期80-84,共5页
Acta Microbiologica Sinica
基金
国家自然科学基金
关键词
空肠弯曲菌
细胞紧张性肠毒素
理化特性
Campylobacter jejuni,Cytotonic enterotoxin,Physicochemical properties
