摘要
目的探讨支气管扩张不同时期炎症因子LT-B4和TNF-α的含量变化及与肺功能的相关性。方法采用ELISA法和诱导痰液检查法分别测定支气管扩张急性加重期和稳定期及健康成人的血清及痰液中LT-B4和TNF-α含量,并测定3组研究对象的肺功能。结果支气管扩张急性加重期血清及痰液中的LT-B4和TNF-α含量均高于稳定期,差异有统计学意义(P<0.05);支气管扩张稳定期血清及痰液中的LT-B4和TNF-α含量均高于正常对照组,差异有统计学意义(P<0.05);支气管扩张急性加重期和稳定期血清及痰液中的LT-B4和TNF-α分别与FEV1和FEV1/FVC呈负相关,差异有统计学意义(P<0.05)。结论 (1)LT-B4和TNF-α共同参与了支气管扩张气道炎症反应的全过程;(2)支气管扩张急性加重过程中可能有多种炎症因子参与、启动,并导致肺功能急剧恶化。
Objective To investigate the concentration variation of couleukotriene B4 and tumornecrosis factor -α during different periods of bronchiectasis and its relevance with lung function. Methods The concentrations of LT - 134 and TNF -α in serum and sputum were measured by using enzyme linked immune sorbent assay （ELISA） for patients with bronchiectasis during acute aggravating and stable phases and healthy adults respectively. Results The concentrations of LT - B4 and TNF - ct in serum and sputum during acute aggravating stage were significantly higher than those during stable phase, the differences had statistical significance （ P 〈 0. 05 ）, and the concentrations during stable stage were significantly higher than those of healthy adults, the differences had statistical significance （ P 〈 0. 05 ）. The concentrations of LT - 134 and TNF -α in serum and sputum during acute aggravating stage and stable phase were both correlated negatively with FEV1 and FEV1/FVC, the differences had statistical significance （ P 〈 0. 05 ）. Conclusions （ 1 ） Both LT - B4 and TNF -α play vital role in the inflammatory reaction of air passage of bronchiectasis. （2） A variety of inflammatory cytokines participate in and launch the process of the acute aggravating stage in bronchietasis, and may worsen lung function sharply.
出处
《辽宁医学院学报》
CAS
2013年第3期24-27,共4页
Journal of Liaoning Medical University (LNMU) Bimonthly
