摘要
目的检测正常胃黏膜、肠上皮化生、异型增生及胃癌组织性别决定区Y框蛋白2(Sox2)的表达,探讨Sox2蛋白在胃癌发生、发展过程中可能的作用。方法选取解放军第二五四医院2003—2011年术后病理明确诊断的组织标本125例,包括30例正常胃黏膜组织、20例肠上皮化生组织、24例异型增生组织、51例胃癌组织。采用免疫组织化学法检测组织标本中Sox2蛋白表达。正常胃黏膜、肠上皮化生、异型增生、胃癌组织Sox2蛋白表达率比较采用χ2检验,进一步组间两两比较采用χ2分割法;不同部位肿瘤、不同大小肿瘤、不同分化程度、不同Lauren分型、不同浸润深度、有无淋巴结转移、不同临床分期胃癌组织Sox2蛋白阳性表达率比较采用χ2检验。结果正常胃黏膜、肠上皮化生、异型增生、胃癌组织Sox2蛋白阳性表达率分别为93.3%(28/30)、80.0%(16/20)、75.0%(18/24)、64.7%(33/51)。胃癌组织Sox2蛋白阳性表达率低于正常胃黏膜组织,且差异有统计学意义(χ2=8.325,P<0.05);其余任意两种组织间Sox2蛋白阳性表达率差异均无统计学意义。低分化胃癌组织Sox2蛋白阳性表达率低于高/中分化胃癌组织[47.4%(9/19)与75.0%(24/32)比较],伴淋巴转移胃癌组织Sox2蛋白阳性表达率低于无淋巴转移胃癌组织[36.4%(4/11)与72.5%(29/40)比较],且差异均有统计学意义(χ2=3.986,4.933;均P<0.05)。不同部位肿瘤、不同大小肿瘤、不同Lauren分型、不同浸润深度、不同临床分期胃癌组织Sox2蛋白阳性表达率差异无统计学意义。结论Sox2蛋白表达降低在胃癌发生、胃癌分化、转移中可能发挥作用。
Objective To detect the expression of sex determining region Y-box 2 （Sox2） in normal gastric mucosa, intestinal metaplasia, dysplasia and gastric carcinoma, and to explore its role in the occurence and development of gastric cancer. Methods One hundred and twenty-five cases of surgical resected gastric specimens were collected from the 254＇h Hospital of the People＇s Liberation Army from 2003 to 2011. The expression of Sox2 was detected in 30 cases of normal gastric tissues, 20 cases of intestinal metaplasia, 24 cases of atypical hyperplasia, and 51 cases of gastric cancinoma by using immunohistochemistry. The differences of expressions of Sox2 in the normal gastric mucosa, intestinal metaplasia, dysplasia and gastric cancer lesion were analyzed by means of X2 test. The differences of expressions of Sox2 between different clinical pathological parameters of gastric cancer （ such as tumor location, size, differentiation, Lauren＇s type, invasion depth, lymph node metastasis and clinical stage） were analyzed by means ofx2 test. Results The positive expression of Sox2 was 93.33% （28/30） in the normal gastric mucosa, 80.0% （16/20） in the intestinal metaplasia, 75.0% （18/24） in the dysplasia, 64.7% （33/51） in the gastric carcinoma, respectively. The positive expression of Sox2 in the gastric cancer was significantly lower than that in the normal tissue （X2 = 8. 325, P 〈 0.05 ）. There was no significantly difference in the positive expression of Sox2 between any other two lesions. It was found that the positive expression of Sox2 in the specimen of poor differentiation was significantly lower than that of well/moderate differentiation [ 47.4% （ 9/19 ） vs 75.0 （ 24/32 ） ; X2 = 3.986 ,P 〈 0.05 ]. The positive expression of Sox2 in the specimen with lymph node metastasis was significantly lower than ;that in group without lymph node metastasis [ 36.4% （4/11） vs 72.5% （29/40） ;X2 = 4. 933, P 〈 0.05 ]. There was no significantly difference of the positive expression of Sox2 among the other elinieal parameter＇ s groups （ sueh as tumor location, size, Lauren＇s type, invasion depth and elinical stage）. Conclusion The low-expression of Sox2 maybe play a role in the gastrie carcinogenesis and tumor cell differentiation and metastasis.
出处
《中华消化病与影像杂志（电子版）》
2012年第6期22-25,共4页
Chinese Journal of Digestion and Medical Imageology(Electronic Edition)
关键词
胃肿瘤
性别决定区Y框蛋白2
免疫组织化学
Stomach neoplasms
Sex determining region Y-box 2
Immunohistochemistry
