摘要
首先建立了一种基于表面等离子体共振技术的适配体传感器检测溶菌酶。通过自组装的方法将1,4-苯二硫醇连接于芯片表面,将组装好的芯片用金纳米修饰,最后将溶菌酶适配体固定到修饰好的芯片上。结果表明,在0.2~20μmol/L范围内具有良好的线性关系,线性方程为Y=41.81＋3.60 X,检出限为0.09μmol/L。对人血清样品检测,平均回收率为92.9%~99.6%。
In this work,an aptamer-based assay for the detection of lysozyme by surface plasmon resonance was described.Gold nanoparticles were anchored onto a sensor chip surface which was previously modified with self-assembled monolayers of 1,4benzenedithiol.Lysozyme aptamer was immobilized to the modified gold chip.The results showed that the linear range was from 0.2μmol / L to 20μmol / L,the linear equation was Y = 41.81 ＋ 3.60X,the detection limit was 0.09μmol / L.The proposed method was applied to the determination of lysozyme in human serum samples,the average recoveries were 92.9%-99.6%.
出处
《山西大同大学学报（自然科学版）》
2013年第2期34-36,90,共4页
Journal of Shanxi Datong University(Natural Science Edition)
基金
国家自然科学基金项目[21175085]
山西省自然科学基金项目[2009011015-1]
关键词
表面等离子体共振
溶菌酶
金纳米
适配体
surface plasmon resonance
lysozyme
gold nanoparticles
aptamer
