摘要
目的 建立同时测定西归药材中阿魏酸和佛手柑内酯含量的方法。方法 采用色谱柱为PhenomeneX Gemini-NX C18 110A柱（4.6 mm×250 mm，5 μm）；流动相为甲醇-0.05%磷酸水溶液，梯度洗脱；流速为1.0 mL·min^-1；检测波长为322 nm；柱温为30 ℃。结果 阿魏酸在0.020 1~0.603 μg、佛手柑内酯在0.020 3~0.609 μg内线性关系良好；阿魏酸和佛手柑内酯的平均回收率分别为96.8%（RSD=2.0%）和98.7%（RSD=2.9%）。结论 本方法首次建立了同时测定西归中阿魏酸和佛手柑内酯的HPLC分析方法，结果准确，重复性好，可作为西归药材的质量评价方法。 英文摘要：
OBJECTIVE To simultaneous determine the content of ferulic acid and bergapten in Seselopsis. METHODS The chromatographic separation was performed on a PhenomeneX Gemini-NX C18 110A （250 mm×4.60 mm, 5μm）. The mobile phase was consisted of methanol-0.05% phosphoric acid with gradient elute, the flow rate was 1.0 mL·min-1. The detection wavelength was 322 nm and the column temperature was 30 ℃. RESULTS Good linear relationship between the peak area and content of ferulic acid and bergapten was achieved when the amount was within 0.020 1-0.603 μg, 0.020 3-0.609 μg; the recoveries were 96.8% and 98.7%; RSD were 2.0% and 2.9%. CONCLUSION This is the first time to simultaneous determine ferulic acid and bergapten in Seselopsis. The method is accurate, reproducible and suitable to be used for quality assessment of Seselopsis.
出处
《中国现代应用药学》
CAS
CSCD
2013年第6期648-651,共4页
Chinese Journal of Modern Applied Pharmacy
基金
大理学院应用开发研究基金(YYKF2006-1-1)
