摘要
以龙须菜Gracilaria lemaneiformis为材料,研究了不同质量浓度水杨酸对龙须菜的生长速率、渗透调节物质、抗氧化酶、藻胆蛋白和叶绿素荧光动力学特性等的影响。结果表明,外施水杨酸可以促进龙须菜的生长,其中水杨酸质量浓度为10μg/mL的处理组对促进龙须菜的生长效果最好,其日相对生长速率达到6.92%/d,比对照组的相对生长速率增长了26.3%。在用水杨酸处理后的第3天,水杨酸质量浓度为10μg/mL处理组的各指标达到最大,脯氨酸增长了32.5%、SOD增长了24.3%、藻红蛋白增加了24.6%。同时水杨酸促进了Ca2+和K+离子在龙须菜藻体内的积累速率。用水杨酸处理后,叶绿素荧光参数ΦPSⅡ在处理后的第5天开始增长,比对照组增长了9.6%;Fv/Fo在第5天达到最大值,比对照组增长了5.6%。本研究表明,适当质量浓度的水杨酸有利于提高龙须菜的生长速率,其中水杨酸质量浓度为10μg/mL的处理组对龙须菜的处理效果最好。
To investigate the effects of different mass concentration of exogenous salicylic acid on the marine red algae Gracilaria lemaneiformis,the physiological parameters and the chlorophyll fluorescence characteristics of G.lemaneiformis were studied.Results showed that salicylic acid could increase the growth rate and the 10 μg·mL-1 treatment group was better than other groups.The daily relative growth rate reached to 6.92%/d with a increase of 26.3% compared with the control group.When on the 3rd day,the 10 μg·mL-1 treatment group of salicylic acid reached the maximum value with proline increased 32.5%,SOD increased 24.3%,and phycoerythrin increased 24.6% respectively.It also promoted the accumulation of Ca2＋ and K＋ in G.lemaneiformis.The chlorophyll fluorescence ΦPSⅡbegan to increase on the fifth day after 10 μg·mL-1 treatment of salicylic acid and increased 9.6% compared with the control group.Fv/Fo reached a maximum on the fifth day,and increased 5.6% more than the control group.In conclusion,exogenous salicylic acid can enhance the growth of G.lemaneiformis and the 10 μg·mL-1 treatment group of salicylic acid is the best concentration.
出处
《海洋学研究》
北大核心
2013年第2期78-85,共8页
Journal of Marine Sciences
基金
国家自然科学基金资助项目(31072229)
浙江省钱江人才资助项目(2007R10038)
关键词
龙须菜
水杨酸
生理生化
叶绿素荧光
Gracilaria lemaneiformis
salicylic acid
physiology and biochemical
chlorophyll fluorescence
