摘要
采用正相氧化铝柱层析和反相C18柱层析从东北红豆杉培养细胞浸提物中分离纯化了紫杉醇。优化了氧化铝柱层析和反相柱层析的操作条件。实验发现,经过氧化铝柱层析后,测得的紫杉醇量大大增加。经两步层析,使紫杉醇的含量从小于10%提高到95%,样品中微量杂质继以重结晶步骤除去,即可获得纯度超过98%的紫杉醇晶体。采用13CNMR对晶体分析,所得产物结构与文献上紫杉醇的结构一致。
A method is described for separation and purification of taxol from the extract of Taxus cuspidate callus culture with Al 2O 3 normal phase chromatography and C 18 reversed phase chromatography,The operating conditions of the two chromatographic steps were investigated and optimized.The content of taxol was greatly increased after Al 2O 3 chromatography.The purity of taxol reached 95% from less than 1.0% after the two chromatographic steps.The final purity of taxol was more than 98% after the process of crystallization.The 13 CNMR spectrum of the product was identical to that of taxol reported in the literature.
出处
《生物工程学报》
CAS
CSCD
北大核心
2000年第1期69-73,共5页
Chinese Journal of Biotechnology
基金
国家自然科学基金!重点项目 ( 2 973 6 180 )
关键词
紫杉醇
分离
纯化
抗癌药物
柱层析
Taxol,normal phase column chromatography,separation and purification
